Research Article
A Chemiluminescent Immunoassay for Osteocalcin in Human Serum and a Solution to the “Hook Effect”
Table 1
Osteocalcin concentrations in serum samples after several dilutions.
| | Dilution | Sample 1 | Sample 2 | Sample 3 | | ng/mL |
| | None | 63.5 | 96.5 | 67.8 | | 1 : 1 | 85.6 | 154.7 | 113.4 | | 1 : 3 | 74.1 | 141.1 | 102.2 | | 1 : 5 | 51.4 | 90.1 | 69.1 | | 1 : 7 | 29.8 | 56.5 | 42.3 | | 1 : 15 | 16.2 | 32.0 | 23.6 |
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