Research Article
A Chemiluminescent Immunoassay for Osteocalcin in Human Serum and a Solution to the “Hook Effect”
| | BGP concentration (ng/mL) | Samples | Added concentration | Tested | Expected | Recovery (%) |
| 1 | 0.00 | 19.98 | 21.03 | 105.26 | 15.63 | 35.57 | 34.93 | 98.20 | 31.25 | 49.39 | 49.95 | 101.13 | 62.50 | 81.42 | 83.48 | 102.53 | 125.00 | 136.48 | 144.23 | 105.68 |
| 2 | 0 | 44.76 | 45.80 | 102.32 | 15.63 | 59.18 | 61.04 | 103.14 | 31.25 | 78.32 | 75.60 | 96.53 | 62.50 | 105.01 | 105.39 | 100.36 | 125.00 | 157.39 | 168.64 | 107.15 |
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