Pharmacokinetic Comparisons of Different Combinations of Yigan Jiangzhi Formula in Rats: Simultaneous Determination of Fourteen Components by UPLC-MS/MS

Table 4

Accuracy and precision of the fourteen analytes in rat plasma (mean ± SD, n = 3 days, 6 replicates per day).

No.	Analyte	Conc. (ng/mL)	Intraday			Interday
			Measured (ng/mL)	Acc. (%)	Prec. (%)	Measured (ng/mL)	Acc. (%)	Prec. (%)
1	Daidzein	0.50	0.48 ± 0.04	−4.00	8.33	0.48 ± 0.04	−4.00	8.33
		10.00	10.03 ± 1.07	0.30	10.67	10.56 ± 0.75	5.60	7.10
		80.00	81.32 ± 1.59	1.65	1.96	83.14 ± 2.17	3.93	2.61
2	Fermononetin	1.25	1.32 ± 0.04	5.60	3.03	1.22 ± 0.12	−2.40	9.84
		25.00	26.12 ± 0.95	4.48	3.64	26.27 ± 0.88	5.08	3.35
		200.00	209.67 ± 5.14	4.83	2.45	214.35 ± 8.29	7.18	3.87
3	Apigenin	0.5	0.53 ± 0.05	6.00	9.43	0.51 ± 0.05	2.00	9.80
		10	10.13 ± 0.32	1.30	3.16	9.86 ± 0.37	−1.40	3.75
		80	82.42 ± 2.20	3.03	2.45	80.41 ± 3.39	0.51	4.22
4	Luteolin	2.5	2.65 ± 0.09	6.00	3.40	2.51 ± 0.27	0.40	10.76
		50	52.03 ± 1.42	4.06	2.73	52.18 ± 1.56	4.36	2.99
		400	426.65 ± 8.57	6.66	2.01	408.59 ± 16.33	2.15	4.00
5	Berberine	0.5	0.48 ± 0.04	−4.00	8.33	0.49 ± 0.06	−2.00	12.24
		10	10.83 ± 0.34	8.30	3.14	10.59 ± 0.92	5.80	8.69
		80	86.55 ± 1.31	8.19	1.51	83.71 ± 3.11	4.64	3.72
6	Jatrorrhizin	0.5	0.53 ± 0.04	6.00	7.55	0.52 ± 0.05	4.00	9.62
		10	10.68 ± 0.19	6.80	1.78	10.95 ± 0.52	9.50	4.75
		80	77.22 ± 1.67	−3.48	2.16	77.54 ± 1.97	−3.07	2.54
7	Palmatine	0.5	0.52 ± 0.04	4.00	7.69	0.55 ± 0.05	10.00	9.09
		10	10.37 ± 0.23	3.70	2.22	10.54 ± 0.62	5.40	5.88
		80	79.30 ± 1.08	−0.88	1.36	80.02 ± 3.02	0.02	3.77
8	Puerarin	5	5.11 ± 0.47	2.20	9.20	5.23 ± 0.68	4.60	13.60
		100	102.31 ± 7.76	2.31	7.58	104.50 ± 6.69	4.50	6.40
		800	778.29 ± 16.52	−2.71	2.12	782.42 ± 18.26	−2.20	2.33
9	Ononin	1.25	1.27 ± 0.08	1.60	6.30	1.28 ± 0.11	2.40	8.59
		25	25.87 ± 0.76	3.48	2.94	25.70 ± 0.62	2.80	2.41
		200	185.75 ± 5.04	−7.13	2.71	178.21 ± 7.76	−10.90	4.35
10	Calycosin-7-O-β-D-Glc	0.5	0.48 ± 0.04	−4.00	8.33	0.51 ± 0.04	2.00	7.84
		10	10.03 ± 0.18	0.30	1.79	9.65 ± 0.46	−3.50	4.77
		80	80.43 ± 1.38	0.54	1.72	74.57 ± 5.46	−6.79	7.32
11	Tanshinol	25	26.35 ± 2.71	5.40	10.28	24.63 ± 2.26	−1.48	9.18
		500	506.70 ± 13.32	1.34	2.63	475.29 ± 30.29	−4.94	6.37
		4000	3786.47 ± 111.61	−5.34	2.95	3691.07 ± 146.59	−7.72	3.97
12	Rosmarinic acid	1.25	1.18 ± 0.08	−5.60	6.78	1.20 ± 0.07	−4.00	5.83
		25	23.63 ± 0.57	−5.48	2.41	23.05 ± 1.06	−7.80	4.60
		200	220.90 ± 4.96	10.45	2.25	215.64 ± 12.18	7.82	5.65
13	Alkanoic acid	5	5.02 ± 0.55	0.40	10.96	4.79 ± 0.49	−4.20	10.23
		100	100.22 ± 2.23	0.22	2.23	94.08 ± 5.46	−5.92	5.80
		800	758.75 ± 26.20	−5.16	3.45	749.11 ± 22.27	−6.36	2.97
14	Salvianolic acid B	2.5	2.72 ± 0.08	8.80	2.94	2.44 ± 0.25	−2.40	10.25
		50	52.00 ± 0.82	4.00	1.58	48.57 ± 3.18	−2.86	6.55
		400	406.38 ± 5.96	1.60	1.47	404.17 ± 14.87	1.04	3.68