Pharmacokinetic Comparisons of Different Combinations of Yigan Jiangzhi Formula in Rats: Simultaneous Determination of Fourteen Components by UPLC-MS/MS

Table 5

Extraction recovery and matrix effect of the fourteen analytes and two internal standards in rat plasma (mean ± SD, n = 6).

No.	Analyte	Conc. (ng/mL)	Extraction recovery	RSD (%)	Matrix effect	RSD (%)
1	Daidzein	0.5	98.11 ± 5.64	5.75	106.77 ± 8.84	8.28
		10	105.24 ± 7.57	7.19	103.41 ± 6.04	5.84
		80	96.40 ± 2.69	2.79	99.75 ± 2.65	2.66
2	Fermononetin	1.25	88.58 ± 4.13	4.66	96.12 ± 7.71	8.02
		25	90.70 ± 6.63	7.31	92.17 ± 5.20	5.64
		200	91.50 ± 3.22	3.52	94.97 ± 8.94	9.41
3	Apigenin	0.5	94.69 ± 5.12	5.41	90.62 ± 7.89	8.71
		10	102.92 ± 4.14	4.02	94.48 ± 1.69	1.79
		80	87.33 ± 2.47	2.83	98.42 ± 2.46	2.50
4	Luteolin	2.5	74.65 ± 8.93	11.96	94.35 ± 4.82	5.11
		50	77.32 ± 3.36	4.35	94.32 ± 4.98	5.28
		400	74.67 ± 0.94	1.26	92.93 ± 1.17	1.26
5	Berberine	0.5	99.45 ± 4.34	4.37	92.95 ± 8.82	9.49
		10	96.10 ± 3.97	4.13	89.92 ± 2.91	3.23
		80	97.45 ± 3.43	3.52	88.10 ± 1.24	1.41
6	Jatrorrhizin	0.5	108.92 ± 3.05	2.80	89.39 ± 2.11	2.36
		10	97.06 ± 3.79	3.91	80.54 ± 1.37	1.70
		80	96.54 ± 1.93	2.00	93.94 ± 1.79	1.91
7	Palmatine	0.5	92.76 ± 4.93	5.31	101.12 ± 4.91	4.86
		10	97.03 ± 5.38	5.54	91.39 ± 3.63	3.97
		80	99.60 ± 3.04	3.05	92.68 ± 1.57	1.69
8	Puerarin	5	94.45 ± 7.51	7.95	97.14 ± 4.81	4.95
		100	93.55 ± 5.46	5.84	106.56 ± 1.87	1.75
		800	98.28 ± 2.68	2.73	89.56 ± 1.80	2.01
9	Ononin	1.25	104.03 ± 6.38	6.13	82.93 ± 2.29	2.76
		25	104.11 ± 2.28	2.19	81.66 ± 1.16	1.41
		200	98.41 ± 1.38	1.40	89.79 ± 2.29	2.55
10	Calycosin-7-O-β-D-Glc	0.5	98.21 ± 4.75	4.84	71.00 ± 4.58	6.45
		10	104.26 ± 2.43	2.33	73.04 ± 4.41	6.04
		80	96.91 ± 3.98	4.10	77.70 ± 1.51	1.94
11	Tanshinol	25	98.86 ± 6.14	6.21	98.67 ± 1.80	1.82
		500	97.99 ± 3.65	3.72	90.88 ± 2.48	2.73
		4000	102.47 ± 2.03	1.98	87.06 ± 4.37	5.02
12	Rosmarinic acid	1.25	95.45 ± 4.53	4.75	94.79 ± 9.09	9.59
		25	96.60 ± 2.76	2.86	96.08 ± 6.78	7.06
		200	96.92 ± 3.84	3.96	96.80 ± 2.84	2.93
13	Alkanoic acid	5	88.12 ± 7.80	8.85	111.53 ± 8.22	7.37
		100	86.99 ± 4.42	5.08	112.35 ± 6.11	5.44
		800	92.60 ± 2.20	2.38	106.26 ± 2.43	2.29
14	Salvianolic acid B	2.5	85.57 ± 9.14	10.68	111.95 ± 8.63	7.71
		50	83.19 ± 3.37	4.05	114.98 ± 9.97	8.67
		400	83.81 ± 4.66	5.56	112.75 ± 2.83	2.51
IS1	Liquiritigenin	50	93.46	6.18	98.20	4.35
IS2	Hesperidin	50	89.27	3.88	100.79	4.70