Contribution to the Detection of Poor Quality Sildenafil Drugs in Burkina Faso Using High-Performance Thin-Layer Chromatography

Table 2

Optimized operating conditions for the determination of sildenafil.

Parameters	Value/selected characteristic(s)
Mobile phase	Methylene chloride-methanol (9 : 1, v/v)
Solubilization and dilution solvent	Methanol
Plates	Merck TLC silica gel F254 20 × 10 cm
Deposited volume on the plate	5 μL
Width of the deposit strip	7.4 mm
Length between deposits	9.4 mm
Distance from the deposition line to the edges of the plate (abscissa and ordinate)	8 and 10 mm
Development distance on plate	70 mm
Development mode	Linear ascending mode
Saturation time of mobile phase	30 min
Deposit flow	15 µL/s
Deposit mode	Spray
Gas for sample application	Nitrogen
Detection wavelength	305 nm