Simultaneous Quantification of Four Ginsenosides in Rat Plasma and Its Application to a Comparative Pharmacokinetic Study in Normal and Depression Rats Using UHPLC-MS/MS

Table 1

Optimized parameters for the detection of G-Rh₁, G-Rb₁, G-Rc, G-Rd, and IS.

Analytes	Retention time (min)	Detect mode	Precursor	Product	Collision energy (eV)	RF lens (V)	Fragmentor voltage (V)
G-Rh1	4.75	ESI	637.3583	475.5166	40.287	198.371	175
G-Rb1	8.70	ESI	1107.5560	945.5067; 783.4598; 621.4125	55.000	259.652	220
G-Rc	10.14	ESI	1077.5464	945.4226; 783.4203; 621.2607; 459.5873	49.792	247.820	175
G-Rd	12.51	ESI	945.4976	783.8463; 621.5194; 459.2995	45.646	276.337	220
G-Rg3 (IS)	13.28	ESI	783.4890	621.4325; 459.3777	41.449	298.483	175