Simultaneous Quantification of Four Ginsenosides in Rat Plasma and Its Application to a Comparative Pharmacokinetic Study in Normal and Depression Rats Using UHPLC-MS/MS

Table 4

Stabilities of G-Rh₁, G-Rb₁, G-Rc, and G-Rd under various storage conditions (n = 6).

Analytes	Concentration (ng/mL)	Short term (24 h, room temperature)		Long term (15 days, −20°C)		Freeze-thaw (3 cycles)		Postpreparative (8 h, 4°C)
		RE (%)	RSD (%)	RE (%)	RSD (%)	RE (%)	RSD (%)	RE (%)	RSD (%)
G-Rh1	25	4.78	3.69	−3.78	0.58	1.04	4.13	5.46	1.65
	250	−4.64	4.57	−7.34	7.66	3.48	3.62	3.87	3.64
	2500	3.75	2.56	6.66	5.93	4.86	0.78	5.19	4.79
G-Rb1	25	1.76	4.87	1.22	6.21	5.87	4.42	5.55	7.03
	250	−2.24	1.02	2.22	0.75	−9.34	2.77	2.74	3.25
	2500	2.08	3.62	−3.06	4.24	3.98	2.95	−7.34	1.58
G-Rc	25	−3.43	4.02	4.43	4.89	−6.43	4.93	−4.39	5.63
	250	−6.34	4.38	6.32	7.36	−5.65	4.7	4.53	1.07
	2500	2.08	1.66	8.15	3.18	4.01	4.89	3.75	0.73
G-Rd	25	3.98	5.42	4.83	3.28	5.43	3.34	−6.3	5.79
	250	−7.43	0.72	−1.37	3.33	−8.43	4.72	7.64	3.85
	2500	−3.87	2.09	2.65	3.23	−1.75	4.05	3.85	0.17