Development of Simple Analytical Method for B-Group Vitamins in Nutritional Products: Enzymatic Digestion and UPLC-MS/MS Quantification

Table 1

Basic parameters of the LC-MS/MS system in this study.

Parameter	Mode and setting value
Mobile phase A	Ammonium formate in water
Mobile phase B	Methanol
Flow rate	0.15 mL/min
Injection volume	10 µL
Column temperature	30°C
Ionization	Electrospray ionization in positive mode (ESI+)
Capillary voltage	2500 V
Desolvation gas temperature	500°C
Desolvation gas flow	800 L/h
Cone gas flow	150 L/h
Collision gas flow	0.15 mL/min
Nebulizer	7 bar
Acquisition	Multiple reaction monitoring (MRM)