Development of Simple Analytical Method for B-Group Vitamins in Nutritional Products: Enzymatic Digestion and UPLC-MS/MS Quantification

Table 3

MS/MS parameters for the determination of B-group vitamins in this study (bold numbers indicate quantitative ions).

Compound	Precursor ion (m/z)	Product ion (m/z)	Cone voltage (V)	Collision energy (eV)
THI	265	81	20	30
		122	24	34
^13C4-THI	269	81	24	28
		122	22	16
RIB	377	198	34	38
		243	34	22
13C4^15N2-RIB	383	175	44	38
		249	44	22
NIC	123	80	44	16
		96	44	18
^2H4-NIC	127	84	44	18
		100	44	18
NIA	124	80	40	20
		106	40	20
^2H4-NIA	128	84	44	16
		109	44	16
PYN	170	134	30	20
		152	30	13
^13C4-PYN	174	138	28	20
		156	28	12
PYL	168	94	24	22
		150	24	12
^2H3-PYL	171	97	22	24
		153	22	12
PYM	169	134	24	20
		152	24	12
^2H3-PYM	172	136	26	20
		155	26	14