Development and Validation of a Stability-Indicating HPLC Method for the Simultaneous Determination of trans-Resveratrol and cis-Resveratrol in an Injectable Solution

Table 2

Results of the forced degradation study of t-RSV samples: STD, F1, and F2.

Condition		T (°C)	STD				F1				F2
			KA (×10^−3 h^−1)	t90 (h)	r^2	% recovery (120 h)	KA (×10^−3 h^−1)	t90 (h)	r^2	% recovery (120 h)	KA (×10^−3 h^−1)	t90 (h)	r^2	% recovery (120 h)
Acid hydrolysis, 0.1 N HCl		40	0.60	174.91	0.91	88.06 ± 0.42	14.95	7.02	0.56	12.89 ± 0.46	41.87	2.51	0.75	36.58 ± 0.64^a
		60	1.35	77.89	0.99	81.06 ± 0.23	17.95	5.85	0.98	12.03 ± 0.64	5.20	20.20	0.91	52.53 ± 0.14
Basic hydrolysis, 0.1 N NaOH		40	42.75	2.46	0.74	4.41 ± 1.839^b	68.17	1.54	0.62	19.44 ± 1.40^a	36.30	2.90	0.76	41.39 ± 1.04^a
		60	119.55	0.88	0.50	5.49 ± 1.21^a	181.05	0.58	0.47	1.30 ± 1.47^a	479.50	0.22	0.46	0.00 ± 0.43^a
Oxidation, 3% H2O2		40	14.88	7.06	0.99	15.43 ± 1.15	6.35	16.54	0.86	43.52 ± 0.28	10.57	9.93	0.96	29.42 ± 0.70
		60	51.29	2.05	0.69	28.12 ± 0.82^a	70.57	1.49	0.61	18.35 ± 0.61^a	383.88	0.27	0.46	0.01 ± 0.34^a
Temperature		4	0.19	540.24	0.99	98.06 ± 0.53	0.23	457.94	0.86	95.37 ± 0.66	0.16	650.77	0.94	97.23 ± 0.76
		25	0.72	146.77	0.89	91.27 ± 0.46	1.32	79.68	0.98	88.76 ± 0.42	1.07	97.99	0.96	93.53 ± 0.32
		40	1.63	64.40	0.95	70.79 ± 1.92	3.98	26.38	0.98	75.84 ± 0.40	3.55	29.60	0.97	65.23 ± 0.91
		60	4.36	24.09	0.97	67.76 ± 0.69	14.89	7.05	0.99	4.40 ± 0.84	14.80	7.10	0.81	8.31 ± 0.50
Photolysis	Darkness	25	0.15	701.17	0.90	97.48 ± 0.60	1.19	87.93	0.86	85.02 ± 0.60	1.46	71.76	0.96	84.47 ± 0.98
	Environment		1.16	90.51	0.86	85.60 ± 0.98	1.23	85.35	0.95	84.95 ± 1.21	7.93	13.24	0.82	36.51 ± 0.73
	UV light λ = 365 nm		14.11	7.43	0.51	12.26 ± 1.07	1.50	6.98	0.51	11.19 ± 0.40	9.02	11.64	0.84	30.35 ± 0.44

K_A = adjusted degradation constant; t₉₀ = 10% RSV degradation time; r² = determination coefficient; % recovery = mean ± standard deviation (n = 3). ^at-RSV concentration recovery after 24 h; ^bt-RSV concentration recovery after 72 h.