Single Standard Substance for the Simultaneous Determination of Eleven Components in the Extract of Paeoniae Radix Alba (Root of Paeonia lactiflora Pall.)

Table 6

The effects of column temperatures, flow rates, columns, and instruments on the relative correction factors.

Relative correction factor	Temp-25^a	Temp-26^a	Temp-28^a	Temp-29^a	Flow-0.90^b	Flow-0.95^b	Flow-1.02^b	Column-YMC^c	Column-BEH^d	Instrument-A^e	Instrument-U^f
fgallic acid/paeoniflorin	0.0361	0.0365	0.0349	0.0350	0.0357	0.0359	0.0354	0.0329	0.0323	0.0359	0.0364
foxypaeoniflorin/paeoniflorin	0.0610	0.0622	0.0620	0.0625	0.0614	0.0625	0.0618	0.0564	0.0548	0.0598	0.0591
fcatechin/paeoniflorin	0.154	0.157	0.154	0.153	0.147	0.156	0.150	0.145	0.143	0.155	0.146
falbiflorin/paeoniflorin	1.10	1.10	1.12	1.14	1.14	1.13	1.09	1.05	1.04	1.15	1.16
fethyl gallate/paeoniflorin	0.0337	0.0334	0.0323	0.0319	0.0335	0.0332	0.0328	0.0310	0.0319	0.0323	0.0330
fgalloylpaeoniflorin/paeoniflorin	0.104	0.103	0.100	0.0995	0.102	0.102	0.102	0.104	0.0999	0.102	0.100
fpentagalloylglucose/paeoniflorin	0.0423	0.0420	0.0409	0.0411	0.0423	0.0419	0.0417	0.0421	0.0435	0.0423	0.0439
fbenzoic acid/paeoniflorin	0.203	0.199	0.199	0.198	0.200	0.194	0.197	0.190	0.198	0.198	0.194
fbenzoylpaeoniflorin/paeoniflorin	0.639	0.634	0.603	0.608	0.608	0.615	0.624	0.601	0.601	0.615	0.619
fpaeonol/paeoniflorin	0.0212	0.0212	0.0203	0.0210	0.0206	0.0207	0.0211	0.0201	0.0207	0.0206	0.0208

^aThe column temperature was changed to 25, 26, 28, or 29°C. ^bThe flow rate was changed to 0.90, 0.95, or 1.02 mL/min. ^cThe column was changed to a YMC-Pack Pro C18 column (5 μm, 250 × 4.6 mm) and the flow rate was changed to 0.95 mL/min. ^dThe column was changed to a Waters BEH C18 column (5 μm, 250 × 4.6 mm) and the flow rate was changed to 0.95 mL/min. ^eThe instrument was changed to an Agilent 1260 system (Agilent Technologies Inc., Waldbornn, Germany). ^fThe instrument was changed to a Dionex UltiMate 3000 system (Thermo Scientific Inc., Germering, Germany).