A Practical Method for Determination of Nine Nucleosides in Tricholoma matsutake by UPLC/MS and Quantitative Analysis of Multicomponents Using Single Marker Method

Table 3

Relative correction factors (RCFs) of nine nucleosides with different analytical columns.

UPLC system	Analytical column	RCFs
		C	U	dU	I	G	dG	dC	A	dA
Waters UPLC I class	BEH C18	0.129	0.447	0.126	1.925	1	0.636	0.099	1.598	0.107
	SB-C18	0.121	0.438	0.122	2.002	1	0.684	0.102	1.594	0.109
	XR-ODS	0.128	0.480	0.129	1.876	1	0.688	0.107	1.491	0.114
Mean		0.126	0.455	0.125	1.934		0.669	0.103	1.561	0.110
SD		0.004	0.022	0.003	0.063		0.029	0.004	0.061	0.004
RSD		3.45	4.87	2.67	3.28		4.28	3.99	3.90	3.40