A Practical Method for Determination of Nine Nucleosides in Tricholoma matsutake by UPLC/MS and Quantitative Analysis of Multicomponents Using Single Marker Method

Table 4

The results of method validation.

Analyte	Regression equation; (y = ax + b, r^2)	Linear range (ng/mL)	LOD (ng)	LOQ (ng)	Precision (RSD, n = 6)		Repeatability (n = 6)		Recovery (n = 6)
					Intraday (%)	Interday (%)	Mean (μg/g)	RSD (%)	Mean (%)	RSD (%)
G	y = 6564.29x − 354.886, 0.9998	0.9868–98.68	0.3	0.9	2.73	4.08	152.1	0.91	101.9	2.10
A	y = 9880.28x + 530.894, 0.9991	1.1152–111.52	0.4	1.1	1.26	1.30	108.7	0.61	91.1	2.45
U	y = 2565.74x + 890.19, 0.9983	1.2–120	0.4	1.2	0.32	0.86	4.2	2.45	88.4	3.78
C	y = 772.496 x + 1260.27, 0.996	5.742–574.2	1.9	5.7	0.47	1.91	36.9	1.41	97.0	1.76
I	y = 11955x + 391.889, 0.9989	0.9276–92.76	0.3	0.9	0.55	1.57	187.0	0.74	95.9	2.24
dG	y = 4320.97x − 506.873, 0.9997	0.8768–87.68	0.3	0.9	0.78	2.14	9.6	1.42	90.9	2.60
dA	y = 584.119 x + 974.144, 0.9918	5.752–575.2	1.9	5.7	1.41	3.12	13.5	2.38	89.0	2.81
dU	y = 839.666 x + 943.893, 0.9965	4.58–458	1.5	4.5	2.80	3.22	44.9	0.99	104.7	2.01
dC	y = 548.202 x + 664.266, 0.9930	4.402–440.2	1.5	4.5	0.58	2.37	6.6	2.47	89.9	2.52