Method Validation and Characterization of Red Pigment in Beta vulgaris Peels and Pomaces by HPLC-UV and UHPLC-MS/MS
Table 2
Intraday and interday repeatability at various concentrations.
Intraday repeatability
Interday repeatability
#
Standard sample concentration (ppm)
Retention time, tRa (min)
Peak area
Quantity estimates (mg/g)
Standard sample concentration (ppm)
Retention time, tRa (min)
Peak area
Quantity estimates (mg/g)
1
10
7.685 ± 0.02
12181 ± 0.01
10.73 ± 0.17
10
7.588 ± 0.42
14029 ± 0.67
10.95 ± 0.06
2
15
7.680 ± 0.01
19543 ± 0.03
11.61 ± 0.02
15
7.592 ± 0.53
19682 ± 0.04
11.62 ± 0.01
3
20
7.602 ± 0.13
37832 ± 0.14
13.78 ± 0.06
20
7.612 ± 0.03
19756 ± 0.l1
11.63 ± 0.87
4
27
7.692 ± 0.25
37004 ± 0.23
13.68 ± 0.22
27
7.794 ± 0.07
37098 ± 0.03
13.69 ± 0.34
5
35
7.689 ± 0.33
37831 ± 0.16
13.77 ± 0.01
35
7.691 ± 0.14
50082 ± 0.25
15.24 ± 0.05
Mean,
7.670 ± 0.15
28878 ± 0.11
12.71 ± 0.10
7.655 ± 0.24
28129 ± 0.22
12.63 ± 0.27
Results are the mean values from five replicates ± S.D. The different letter “a” means that the tR of individual concentrations and days are statistically different.
