Tissue Distribution of Total Flavonoids Extracts of Drynariae Rhizoma in Young and Old Rats by UPLC–MS/MS Determination

Table 11

Recovery and matrix effect study of luteolin-7-O-β-D-glucoside in rat tissues (n = 6).

Tissues	Spiked (ng/mL)	Recovery		Matrix effects
		Mean (%) ± SD	RSD (%)	Mean (%) ± SD	RSD (%)
Heart	5.05	100.87 ± 12.35	12.24	102.97 ± 11.06	10.74
	10.10	99.96 ± 10.70	10.70	97.19 ± 14.26	14.67
	25.25	90.02 ± 9.44	10.49	92.67 ± 12.21	13.18
Liver	5.05	94.93 ± 14.20	14.96	97.94 ± 13.43	13.71
	10.10	92.00 ± 10.76	11.70	89.94 ± 10.10	11.23
	25.25	95.58 ± 10.45	10.93	100.58 ± 6.20	6.16
Spleen	5.05	93.39 ± 13.76	14.73	95.60 ± 12.14	12.70
	10.10	106.57 ± 14.55	13.65	107.84 ± 14.56	13.50
	25.25	104.63 ± 5.39	5.15	101.16 ± 5.34	5.28
Lung	8.10	107.38 ± 8.33	7.76	107.83 ± 7.81	7.24
	40.50	95.09 ± 12.26	12.79	92.55 ± 9.01	9.74
	81.00	101.05 ± 3.99	3.95	102.4 ± 4.14	4.04
Kidney	5.05	96.75 ± 13.77	14.23	95.49 ± 13.13	13.75
	10.10	102.34 ± 14.32	13.99	106.46 ± 14.21	13.35
	25.25	98.30 ± 10.01	10.18	99.87 ± 10.25	10.26
Brain	5.05	93.82 ± 8.47	9.03	94.06 ± 8.78	9.33
	10.10	106.65 ± 13.04	12.23	99.03 ± 12.31	12.43
	25.25	100.51 ± 11.27	11.21	98.89 ± 13.15	13.30
Stomach	8.10	94.52 ± 4.07	4.31	97.78 ± 10.50	10.74
	40.50	100.84 ± 11.08	10.99	96.03 ± 5.00	5.21
	81.00	101.98 ± 6.28	6.16	101.12 ± 7.08	7.00
Uterus	5.05	88.08 ± 10.75	12.20	93.66 ± 11.69	12.48
	10.10	89.52 ± 4.55	5.08	89.29 ± 4.69	5.25
	25.25	92.2 ± 8.76	9.50	96.70 ± 12.98	13.42
Ovary	5.05	91.09 ± 12.84	14.10	92.83 ± 12.48	13.44
	10.10	97.72 ± 13.49	13.80	94.93 ± 14.17	14.93
	25.25	95.52 ± 3.65	3.82	96.21 ± 4.98	5.18
Intestine	8.10	97.78 ± 10.52	10.76	99.04 ± 12.78	12.90
	40.50	98.43 ± 13.42	13.63	92.34 ± 8.89	9.63
	81.00	99.19 ± 12.22	12.32	99.90 ± 12.97	12.98