Tissue Distribution of Total Flavonoids Extracts of Drynariae Rhizoma in Young and Old Rats by UPLC–MS/MS Determination

Table 12

Recovery and matrix effect study of astragalin in rat tissues (n = 6).

Tissues	Spiked (ng/mL)	Recovery		Matrix effects
		Mean (%) ± SD	RSD (%)	Mean (%) ± SD	RSD (%)
Heart	8.10	96.05 ± 10.66	11.10	101.93 ± 13.66	13.40
	40.50	93.71 ± 8.42	8.99	95.41 ± 9.36	9.81
	81.00	104.43 ± 12.29	11.77	103.7 ± 12.04	11.61
Liver	8.10	115.16 ± 14.23	12.36	119.16 ± 14.67	12.31
	40.50	88.31 ± 8.64	9.78	93.07 ± 12.34	13.26
	81.00	100.5 ± 5.09	5.06	103.75 ± 6.12	5.90
Spleen	5.05	93.31 ± 9.96	10.67	87.88 ± 11.81	13.44
	10.10	118.57 ± 10.60	8.94	112.75 ± 14.93	13.24
	25.25	94.99 ± 14.01	14.75	95.34 ± 13.54	14.20
Lung	8.10	109.23 ± 14.92	13.66	108.4 ± 14.06	12.97
	40.50	103.39 ± 7.85	7.59	108.74 ± 9.74	8.96
	81.00	107.62 ± 6.59	6.12	104.04 ± 8.35	8.03
Kidney	5.05	94.5 ± 12.84	13.59	95.33 ± 11.54	12.11
	10.10	99.5 ± 12.95	13.02	104.44 ± 11.11	10.64
	25.25	93.96 ± 13.12	13.96	94.98 ± 13.52	14.23
Brain	5.05	93.98 ± 11.22	11.94	97.5 ± 13.92	14.28
	10.10	89.98 ± 8.59	9.55	92.55 ± 12.47	13.47
	25.25	89.2 ± 8.53	9.56	94.42 ± 11.88	12.58
Stomach	8.10	112.99 ± 14.09	12.47	108.96 ± 14.43	13.24
	81.00	108.37 ± 9.73	8.98	105.71 ± 11.12	10.52
	162.00	97.94 ± 12.88	13.15	98.35 ± 12.24	12.45
Uterus	5.05	91.17 ± 7.91	8.68	96.04 ± 13.06	13.60
	10.10	96.22 ± 11.18	11.62	101.13 ± 14.18	14.02
	25.25	100.08 ± 12.77	12.76	103.07 ± 14.14	13.72
Ovary	8.10	108.74 ± 13.50	12.41	116.05 ± 13.30	11.46
	40.50	104.44 ± 14.80	14.17	107.02 ± 13.64	12.75
	81.00	109.00 ± 14.30	13.12	104.89 ± 14.40	13.73
Intestine	8.10	109.56 ± 14.08	12.85	111.85 ± 14.31	12.79
	40.50	95.85 ± 13.47	14.05	96.94 ± 12.25	12.64
	81.00	99.52 ± 10.07	10.12	100.56 ± 11.45	11.39