Tissue Distribution of Total Flavonoids Extracts of Drynariae Rhizoma in Young and Old Rats by UPLC–MS/MS Determination

Table 16

Recovery and matrix effect study of kaempferol in rat tissues (n = 6).

Tissues	Spiked (ng/mL)	Recovery		Matrix effects
		Mean (%) ± SD	RSD (%)	Mean (%) ± SD	RSD (%)
Liver	8.10	101.65 ± 14.31	14.08	106.96 ± 14.12	13.20
	40.50	115.4 ± 14.20	12.31	121.79 ± 10.89	8.94
	81.00	102.17 ± 14.31	14.01	104.84 ± 13.72	13.09
Spleen	12.20	93.16 ± 12.41	13.32	86.15 ± 6.77	7.86
	91.50	102.12 ± 10.97	10.74	108.20 ± 14.34	13.25
	183.00	103.48 ± 14.72	14.22	99.06 ± 10.94	11.04
Lung	5.05	100.12 ± 13.76	13.74	96.20 ± 6.49	6.75
	10.10	87.78 ± 6.08	6.93	91.86 ± 13.12	14.28
	25.25	94.43 ± 13.83	14.65	90.60 ± 12.32	13.60
Kidney	12.20	89.61 ± 9.00	10.04	90.36 ± 7.52	8.32
	42.70	99.04 ± 10.66	10.76	98.81 ± 11.00	11.13
	85.40	103.94 ± 7.94	7.64	102.19 ± 10.66	10.43
Stomach	5.05	95.6 ± 14.05	14.70	95.29 ± 14.16	14.86
	10.10	105.45 ± 14.18	13.45	103.78 ± 14.59	14.06
	25.25	97.72 ± 13.78	14.10	97.81 ± 13.67	13.98
Uterus	5.05	94.69 ± 10.39	10.97	99.45 ± 9.78	9.83
	10.10	105.13 ± 13.83	13.16	98.36 ± 10.66	10.84
	25.25	95.33 ± 12.51	13.12	93.90 ± 11.54	12.29
Intestine	8.10	104.22 ± 13.70	13.15	101.14 ± 13.81	13.65
	121.50	82.72 ± 3.60	4.35	87.29 ± 10.75	12.32
	243.00	110.63 ± 14.41	13.03	112.54 ± 14.91	13.25