Tissue Distribution of Total Flavonoids Extracts of Drynariae Rhizoma in Young and Old Rats by UPLC–MS/MS Determination

Table 17

Stability of neoeriocitrin in rat tissues (n = 6).

Tissues	Spiked (ng/mL)	Room temperature			Freeze-thaw (−20°C, 3 times)			Long term (−20°C, 30 days)
		Mean ± SD	RSD (%)	RE (%)	Mean ± SD	RSD (%)	RE (%)	Mean ± SD	RSD (%)	RE (%)
Heart	8.10	6.95 ± 0.80	11.51	−14.20	8.15 ± 1.18	14.48	0.62	7.07 ± 1.02	14.43	−12.72
	121.50	106.50 ± 6.67	6.26	−12.35	119.40 ± 16.17	13.54	−1.73	111.16 ± 5.83	5.24	−8.51
	243.00	254.81 ± 35.08	13.77	4.86	268.16 ± 21.81	8.13	10.35	249.44 ± 25.84	10.36	2.65
Liver	12.20	10.71 ± 0.95	8.87	−12.21	10.78 ± 1.30	12.06	−11.64	10.90 ± 1.52	13.94	−10.66
	610.00	596.81 ± 18.63	3.12	−2.16	592.53 ± 11.67	1.97	−2.86	586.32 ± 15.63	2.67	−3.88
	3,660.00	3,403.71 ± 343.46	10.09	−7.00	3,313.88 ± 225.54	6.81	−9.46	3,294.94 ± 300.05	9.11	−9.97
Spleen	8.10	9.30 ± 0.68	7.31	14.81	9.23 ± 0.50	5.42	13.95	9.23 ± 0.52	5.63	13.95
	810.00	809.27 ± 16.98	2.10	−0.09	798.45 ± 12.24	1.53	−1.43	806.44 ± 14.65	1.82	−0.44
	4,050.00	3,696.74 ± 532.72	14.41	−8.72	3,922.39 ± 506.99	12.93	−3.15	3,596.82 ± 365.71	10.17	11.19
Lung	12.20	13.51 ± 0.65	4.81	10.74	11.68 ± 0.63	5.39	−4.26	12.56 ± 1.38	10.99	2.95
	1,220.00	1,049.18 ± 151.19	14.41	−14.00	1,064.46 ± 105.31	9.89	−12.75	1,064.00 ± 140.09	13.17	−12.79
	4,880.00	4,693.51 ± 174.71	3.72	−3.82	4,655.35 ± 67.79	1.46	−4.60	4,706.89 ± 163.70	3.48	−3.55
Kidney	8.10	9.27 ± 0.41	4.42	14.44	9.29 ± 0.22	2.37	14.69	9.17 ± 0.31	3.38	13.21
	121.50	130.10 ± 17.12	13.16	7.08	115.37 ± 7.09	6.15	−5.05	117.29 ± 16.56	14.12	−3.47
	243.00	211.85 ± 16.00	7.55	−12.82	268.79 ± 20.77	7.73	10.61	227.65 ± 32.07	14.09	−6.32
Brain	8.10	9.10 ± 0.68	7.47	12.35	8.51 ± 0.98	11.52	5.06	8.51 ± 0.96	11.28	5.06
	40.50	36.84 ± 4.11	11.16	−9.04	35.02 ± 5.17	14.76	−13.53	35.89 ± 4.53	12.62	−11.38
	81.00	91.02 ± 4.71	5.17	12.37	83.99 ± 6.69	7.97	3.69	86.52 ± 7.86	9.08	6.81
Stomach	8.10	8.79 ± 1.10	12.51	8.52	8.03 ± 0.61	7.60	−0.86	8.42 ± 0.55	6.53	3.95
	810.00	849.82 ± 40.54	4.77	4.92	926.35 ± 17.57	1.90	14.36	867.42 ± 68.59	7.91	7.09
	4,050.00	4,342.23 ± 411.23	9.47	7.22	4,039.22 ± 283.82	7.03	−0.27	4,301.44 ± 400.81	9.32	6.21
Uterus	8.10	8.51 ± 1.02	11.99	5.06	9.22 ± 0.82	8.89	13.83	8.14 ± 1.14	14.00	0.49
	121.50	119.97 ± 12.44	10.37	−1.26	118.34 ± 15.89	13.43	−2.60	125.77 ± 17.07	13.57	3.51
	243.00	255.57 ± 29.61	11.59	5.17	211.49 ± 16.06	7.59	−12.97	232.87 ± 16.56	7.11	−4.17
Ovary	8.10	8.06 ± 0.50	6.20	−0.49	7.84 ± 0.84	10.71	−3.21	7.27 ± 0.90	12.38	−10.25
	121.50	111.60 ± 15.68	14.05	−8.15	106.87 ± 9.78	9.15	−12.04	111.12 ± 15.83	14.25	−8.54
	243.00	230.40 ± 30.17	13.09	−5.19	233.50 ± 27.75	11.88	−3.91	207.73 ± 9.56	4.60	−14.51
Intestine	8.10	8.48 ± 1.10	12.97	4.69	9.05 ± 0.45	4.97	11.73	8.62 ± 1.06	12.30	6.42
	810.00	794.00 ± 83.11	10.47	−1.98	772.96 ± 9.97	1.29	−4.57	812.09 ± 62.32	7.67	0.26
	4,050.00	4,361.18 ± 410.40	9.41	7.68	4,111.91 ± 456.07	11.09	1.53	4,607.89 ± 16.92	0.37	13.78