Tissue Distribution of Total Flavonoids Extracts of Drynariae Rhizoma in Young and Old Rats by UPLC–MS/MS Determination

Table 18

Stability of luteolin-7-O-β-D-glucoside in rat tissues (n = 6).

Tissues	Spiked (ng/mL)	Room temperature			Freeze-thaw (−20°C, 3 times)			Long term (−20°C, 30 days)
		Mean ± SD	RSD (%)	RE (%)	Mean ± SD	RSD (%)	RE (%)	Mean ± SD	RSD (%)	RE (%)
Heart	5.05	5.13 ± 0.66	12.87	1.58	5.07 ± 0.59	11.64	0.40	5.05 ± 0.53	10.52	−0.20
	10.10	9.36 ± 0.42	4.49	−7.33	10.12 ± 1.09	10.77	0.20	10.00 ± 0.78	7.80	−0.99
	25.25	22.45 ± 2.22	9.89	−11.09	25.44 ± 1.48	5.82	0.75	23.44 ± 1.91	8.15	−7.17
Liver	5.05	4.56 ± 0.61	13.38	−9.70	5.07 ± 0.25	4.93	0.40	5.06 ± 0.56	11.07	0.20
	10.10	8.77 ± 1.12	12.77	−13.17	9.70 ± 0.72	7.42	−3.96	9.64 ± 0.95	9.85	−4.55
	25.25	22.84 ± 1.38	6.04	−9.54	26.21 ± 1.43	5.46	3.80	23.21 ± 2.62	11.29	−8.08
Spleen	5.05	4.37 ± 0.52	11.90	−13.47	5.04 ± 0.33	6.55	−0.20	4.82 ± 0.62	12.86	−4.55
	10.10	9.78 ± 1.38	14.11	−3.17	11.58 ± 1.17	10.10	14.65	10.16 ± 1.02	10.04	0.59
	25.25	27.07 ± 1.16	4.29	7.21	24.90 ± 1.29	5.18	−1.39	26.63 ± 1.87	7.02	5.47
Lung	8.10	8.82 ± 0.74	8.39	8.89	8.41 ± 0.59	7.02	3.83	8.48 ± 0.85	10.02	4.69
	40.50	39.56 ± 5.82	14.71	−2.32	39.03 ± 3.82	9.79	−3.63	39.16± 5.77	14.73	−3.31
	81.00	82.50 ± 4.39	5.32	1.85	82.39 ± 2.63	3.19	1.72	82.74 ± 4.20	5.08	2.15
Kidney	5.05	4.65 ± 0.33	7.10	−7.92	5.15 ± 0.50	9.71	1.98	4.95 ± 0.29	5.86	−1.98
	10.10	9.15 ± 0.43	4.70	−9.41	11.32 ± 0.67	5.92	12.08	10.23 ± 1.03	10.07	1.29
	25.25	23.42 ± 1.98	8.45	−7.25	26.72 ± 1.28	4.79	5.82	25.76 ± 2.08	8.07	2.02
Brain	5.05	5.01 ± 0.29	5.79	−0.79	4.63 ± 0.52	11.23	−8.32	4.88 ± 0.51	10.45	−3.37
	10.10	10.90 ± 1.49	13.67	7.92	9.90 ± 1.40	14.14	−1.98	11.15 ± 0.78	7.00	10.40
	25.25	26.37 ± 3.36	12.74	4.44	23.03 ± 1.87	8.12	−8.79	24.71 ± 1.25	5.06	−2.14
Stomach	8.10	7.63 ± 0.44	5.77	−5.80	8.25 ± 0.99	12.00	1.85	7.70 ± 0.45	5.84	−4.94
	40.50	43.37 ± 4.04	9.32	7.09	37.46 ± 0.73	1.95	−7.51	42.13 ± 5.48	13.01	4.02
	81.00	79.72 ± 0.23	0.29	−1.58	83.45 ± 7.55	9.05	3.02	80.64 ± 1.53	1.90	−0.44
Uterus	5.05	4.39 ± 0.13	2.96	−13.07	5.12 ± 0.36	7.03	1.39	4.85 ± 0.40	8.25	−3.96
	10.10	9.06 ± 0.64	7.06	−10.30	8.92 ± 0.18	2.02	−11.68	9.19 ± 0.51	5.55	−9.01
	25.25	22.05 ± 1.03	4.67	−12.67	26.33 ± 2.74	10.41	4.28	22.84 ± 0.66	2.89	−9.54
Ovary	5.05	4.70 ± 0.61	12.98	−6.93	4.45 ± 0.66	14.83	−11.88	4.73 ± 0.63	13.32	−6.34
	10.10	10.18 ± 1.02	10.02	0.79	9.05 ± 0.65	7.18	−10.40	9.31 ± 0.61	6.55	−7.82
	25.25	24.37 ± 1.14	4.68	−3.49	24.60 ± 1.54	6.26	−2.57	23.99 ± 1.27	5.29	−4.99
Intestine	8.10	7.82 ± 1.13	14.45	−3.46	8.57 ± 0.95	11.09	5.80	8.13 ± 1.13	13.90	0.37
	40.50	42.25 ± 5.88	13.92	4.32	35.91 ± 1.88	5.24	−11.33	40.48 ± 5.17	12.77	−0.05
	81.00	78.00 ± 9.32	11.95	−3.70	86.10 ± 10.19	11.84	6.30	83.37 ± 11.64	13.96	2.93