Tissue Distribution of Total Flavonoids Extracts of Drynariae Rhizoma in Young and Old Rats by UPLC–MS/MS Determination

Table 19

Stability of astragalin in rat tissues (n = 6).

Tissues	Spiked (ng/mL)	Room temperature			Freeze-thaw (−20°C, 3 times)			Long term (−20°C, 30 days)
		Mean ± SD	RSD (%)	RE (%)	Mean ± SD	RSD (%)	RE (%)	Mean ± SD	RSD (%)	RE (%)
Heart	8.10	7.70 ± 0.45	5.84	−4.94	8.47 ± 1.09	12.87	4.57	8.00 ± 0.93	11.63	−1.23
	40.50	36.34 ± 1.61	4.43	−10.27	40.78 ± 3.39	8.31	0.69	39.21 ± 4.04	10.30	−3.19
	81.00	86.71 ± 12.77	14.73	7.05	82.81 ± 4.93	5.95	2.23	88.04 ± 10.68	12.13	8.69
Liver	8.10	8.40 ± 0.49	5.83	3.70	9.25 ± 0.48	5.19	14.20	9.28 ± 1.20	12.93	14.57
	40.50	38.23 ± 1.64	4.29	−5.60	40.11 ± 5.05	12.59	−0.96	37.31 ± 3.82	10.24	−7.88
	81.00	78.97 ± 1.50	1.90	−2.51	86.85 ± 4.39	5.05	7.22	79.81 ± 2.36	2.96	−1.47
Spleen	5.05	4.83 ± 0.62	12.84	−4.36	4.67 ± 0.52	11.13	−7.52	5.08 ± 0.25	4.92	0.59
	10.10	10.65 ± 0.66	6.20	5.45	10.39 ± 1.08	10.39	2.87	11.05 ± 1.42	12.85	9.41
	25.25	23.18 ± 3.01	12.99	−8.20	26.33 ± 2.81	10.67	4.28	25.37 ± 3.04	11.98	0.48
Lung	8.10	9.00 ± 1.11	12.33	11.11	8.58 ± 0.72	8.39	5.93	8.59 ± 0.91	10.59	6.05
	40.50	41.43 ± 3.85	9.29	2.30	44.81 ± 4.50	10.04	10.64	43.01 ± 3.91	9.09	6.20
	81.00	86.86 ± 7.48	8.61	7.23	83.80 ± 6.72	8.02	3.46	90.25 ± 1.68	1.86	11.42
Kidney	5.05	4.43 ± 0.47	10.61	−12.28	4.90 ± 0.60	12.24	−2.97	4.53 ± 0.53	11.70	−10.30
	10.10	9.49 ± 0.84	8.85	−6.04	10.99 ± 1.25	11.37	8.81	10.40 ± 1.02	9.81	2.97
	25.25	22.06 ± 1.98	8.98	−12.63	26.65 ± 1.89	7.09	5.54	23.32 ± 3.02	12.95	−7.64
Brain	5.05	4.48 ± 0.59	13.17	−11.29	5.44 ± 0.52	9.56	7.72	4.82 ± 0.42	8.71	−4.55
	10.10	9.00 ± 0.76	8.44	−10.89	9.87 ± 1.46	14.79	−2.28	9.59 ± 0.74	7.72	−5.05
	25.25	23.04 ± 2.82	12.24	−8.75	23.76 ± 3.54	14.90	−5.90	21.71 ± 0.54	2.49	−14.02
Stomach	8.10	8.49 ± 0.69	8.13	4.81	9.30 ± 1.33	14.30	14.81	9.01 ± 0.59	6.55	11.23
	81.00	93.14 ± 4.03	4.33	14.99	79.09 ± 1.25	1.58	−2.36	87.46 ± 7.78	8.90	7.98
	162.00	156.45 ± 23.16	14.80	−3.43	152.89 ± 21.65	14.16	−5.62	154.82 ± 22.74	14.69	−4.43
Uterus	5.05	4.37 ± 0.34	7.78	−13.47	5.23 ± 0.49	9.37	3.56	4.52 ± 0.52	11.50	−10.50
	10.10	9.18 ± 0.78	8.50	−9.11	11.12 ± 1.40	12.59	10.10	10.23 ± 1.09	10.65	1.29
	25.25	23.30 ± 2.05	8.80	−7.72	28.41 ± 1.48	5.21	12.51	26.18 ± 3.07	11.73	3.68
Ovary	8.10	9.05 ± 1.07	11.82	11.73	9.15 ± 1.34	14.64	12.96	8.60 ± 1.19	13.84	6.17
	40.50	37.87 ± 4.17	11.01	−6.49	45.76 ± 5.66	12.37	12.99	42.26 ± 6.03	14.27	4.35
	81.00	82.74 ± 12.23	14.78	2.15	92.04 ± 8.01	8.70	13.63	92.04 ± 11.52	12.52	13.63
Intestine	8.10	8.28 ± 1.11	13.41	2.22	8.99 ± 0.78	8.68	10.99	8.85 ± 0.81	9.15	9.26
	40.50	40.39 ± 5.04	12.48	−0.27	36.13 ± 1.00	2.77	−10.79	39.29 ± 5.25	13.36	−2.99
	81.00	83.65 ± 9.85	11.78	3.27	81.48 ± 9.48	11.63	0.59	85.26 ± 7.09	8.32	5.26