Tissue Distribution of Total Flavonoids Extracts of Drynariae Rhizoma in Young and Old Rats by UPLC–MS/MS Determination

Table 20

Stability of naringin in rat tissues (n = 6).

Tissues	Spiked (ng/mL)	Room temperature			Freeze-thaw (−20°C, 3 times)			Long term (−20°C, 30 days)
		Mean ± SD	RSD (%)	RE (%)	Mean ± SD	RSD (%)	RE (%)	Mean ± SD	RSD (%)	RE (%)
Heart	8.10	8.18 ± 0.99	12.10	0.99	9.31 ± 0.59	6.34	14.94	8.34 ± 1.05	12.59	2.96
	405.00	398.76 ± 53.76	13.48	−1.54	363.13 ± 28.56	7.86	−10.34	392.60 ± 57.37	14.61	−3.06
	810.00	829.59 ± 77.67	9.36	2.42	842.66 ± 90.17	10.70	4.03	833.28 ± 79.23	9.51	2.87
Liver	12.20	12.40 ± 1.51	12.18	1.64	11.43 ± 0.64	5.60	−6.31	11.77 ± 1.76	14.95	−3.52
	1,220.00	1,109.92 ± 162.72	14.66	−9.02	1,056.84 ± 59.00	5.58	−13.37	1,148.25 ± 169.62	14.77	−5.88
	4,880.00	4,705.83 ± 702.90	14.94	−3.57	5,273.67 ± 504.56	9.57	8.07	4,969.00 ± 514.84	10.36	1.82
Spleen	8.10	8.85 ± 1.08	12.20	9.26	9.05 ± 1.22	13.48	11.73	8.07 ± 0.85	10.53	−0.37
	810.00	801.57 ± 45.20	5.64	−1.04	848.96 ± 85.32	10.05	4.81	822.63 ± 46.56	5.66	1.56
	4,050.00	4,143.06 ± 577.07	13.93	2.30	3,865.82 ± 370.26	9.58	−4.55	4,201.39 ± 602.33	14.34	3.74
Lung	8.10	9.26 ± 1.01	10.91	14.32	8.77 ± 0.40	4.56	8.27	9.03 ± 1.06	11.74	11.48
	810.00	841.43 ± 64.45	7.66	3.88	849.40 ± 106.55	12.54	4.86	867.33 ± 105.64	12.18	7.08
	4,050.00	3,705.01 ± 526.75	14.22	−8.52	4,317.76 ± 550.38	12.75	6.61	3,697.64 ± 514.32	13.91	−8.70
Kidney	8.10	9.15 ± 1.10	12.02	12.96	8.31 ± 0.48	5.78	2.59	9.31 ± 1.02	10.96	14.94
	810.00	892.39 ± 40.99	4.59	10.17	834.96 ± 117.60	14.08	3.08	846.20 ± 106.88	12.63	4.47
	4,050.00	4,133.40 ± 608.45	14.72	2.06	3,779.16 ± 448.50	11.87	−6.69	3,747.32 ± 516.20	13.78	−7.47
Brain	8.10	8.35 ± 1.01	12.10	3.09	7.92 ± 1.04	13.13	2.22	7.49 ± 0.50	6.68	−7.53
	405.00	444.59 ± 47.61	10.71	9.78	385.42 ± 41.47	10.76	−4.83	424.79 ± 47.09	11.09	4.89
	810.00	882.19 ± 104.39	11.83	8.91	817.76 ± 120.70	14.76	0.96	810.79 ± 121.05	14.93	0.10
Stomach	8.10	8.47 ± 1.21	14.29	4.57	9.19 ± 1.28	13.93	13.46	7.86 ± 0.52	6.62	−2.96
	810.00	790.05 ± 106.47	13.48	−2.46	777.09 ± 71.41	9.19	−4.06	842.24 ± 101.48	12.05	3.98
	8,100.00	8,149.70 ± 564.58	6.93	0.61	7,454.36 ± 368.62	4.95	−7.97	7,672.71 ± 291.70	3.80	−5.28
Uterus	8.10	8.96 ± 0.64	7.14	10.62	9.09 ± 1.25	13.75	12.22	9.14 ± 0.94	10.28	12.84
	1,215.00	1,155.91 ± 151.48	13.10	−4.86	1,358.76 ± 84.48	6.22	11.83	1,266.07 ± 11.09	0.88	4.20
	2,430.00	2,461.22 ± 348.52	14.16	1.28	2,262.54 ± 87.25	3.86	−6.89	2,330.98 ± 332.29	14.26	−4.07
Ovary	8.10	8.06 ± 1.07	13.28	−0.49	9.16 ± 0.74	8.08	13.09	7.97 ± 1.05	13.17	−1.60
	810.00	820.47 ± 26.50	3.23	1.29	895.06 ± 54.93	6.14	10.50	827.50 ± 32.71	3.95	2.16
	4,050.00	3,954.66 ± 490.84	12.41	−2.35	4,095.99 ± 558.04	13.62	1.14	4,080.56 ± 377.08	9.24	0.75
Intestine	8.10	8.19 ± 0.90	10.99	1.11	9.30 ± 1.24	13.33	14.81	7.63 ± 0.13	1.70	−5.80
	810.00	818.79 ± 115.79	14.14	1.09	877.24 ± 105.43	12.02	8.30	845.43 ± 80.79	9.56	4.37
	8,100.00	8,351.44 ± 1,228.10	14.71	3.10	8,229.70 ± 1,151.14	13.99	1.60	8,818.55 ± 1,318.14	14.95	8.87