Tissue Distribution of Total Flavonoids Extracts of Drynariae Rhizoma in Young and Old Rats by UPLC–MS/MS Determination

Table 21

Stability of eriodictyol in rat tissues (n = 6).

Tissues	Spiked (ng/mL)	Room temperature			Freeze-thaw (−20°C, 3 times)			Long term (−20°C, 30 days)
		Mean ± SD	RSD (%)	RE (%)	Mean ± SD	RSD (%)	RE (%)	Mean ± SD	RSD (%)	RE (%)
Liver	8.10	8.74 ± 0.86	9.84	7.90	9.07 ± 1.31	14.44	11.98	7.90 ± 0.98	12.41	−2.47
	40.50	35.14 ± 2.55	7.26	−13.23	41.26 ± 2.48	6.01	1.88	37.37 ± 2.39	6.40	−7.73
	81.00	86.70 ± 8.92	10.29	7.04	81.90 ± 12.13	14.81	1.11	91.19 ± 9.55	10.47	12.58
Spleen	5.05	4.58 ± 0.65	14.19	−9.31	5.23 ± 0.16	3.06	3.56	4.82 ± 0.61	12.66	−4.55
	10.10	11.14 ± 1.33	11.94	10.30	10.87 ± 0.46	4.23	7.62	11.35 ± 1.08	9.52	12.38
	25.25	24.57 ± 2.36	9.61	−2.69	21.75 ± 1.80	8.28	−13.86	24.87 ± 1.86	7.48	−1.50
Lung	8.10	8.67 ± 0.94	10.84	7.04	8.52 ± 1.06	12.44	5.19	8.94 ± 0.99	11.07	10.37
	40.50	44.21 ± 6.02	13.62	9.16	38.47 ± 3.91	10.16	−5.01	40.58 ± 5.08	12.52	0.20
	81.00	74.39 ± 7.67	10.31	−8.16	88.31 ± 8.37	9.48	9.02	81.83 ± 5.22	6.38	1.02
Stomach	8.10	8.07 ± 0.83	10.29	−0.37	9.21 ± 0.21	2.28	13.70	8.95 ± 0.94	10.50	10.49
	81.00	76.31 ± 4.44	5.82	−5.79	87.52 ± 3.29	3.76	8.05	80.47 ± 3.35	4.16	−0.65
	162.00	156.23 ± 14.25	9.12	−3.56	163.44 ± 23.11	14.14	0.89	165.97 ± 24.01	14.47	2.45
Uterus	5.05	5.34 ± 0.79	14.79	5.74	5.15 ± 0.41	7.96	1.98	4.96 ± 0.67	13.51	−1.78
	10.10	10.77 ± 1.36	12.63	6.63	9.56 ± 1.35	14.12	−5.35	10.42 ± 0.87	8.35	3.17
	25.25	24.07 ± 3.23	13.42	−4.67	22.30 ± 2.21	9.91	−11.68	22.60 ± 3.35	14.82	−10.50
Ovary	8.10	8.99 ± 1.10	12.24	10.99	8.83 ± 1.25	14.16	9.01	9.25 ± 0.73	7.89	14.20
	40.50	37.31 ± 1.78	4.77	−7.88	39.47 ± 5.77	14.62	−2.54	40.46 ± 6.01	14.85	−0.10
	81.00	89.65 ± 10.06	11.22	10.68	84.97 ± 6.08	7.16	4.90	86.94 ± 8.45	9.72	7.33
Intestine	8.10	8.73 ± 1.15	13.17	7.78	8.95 ± 1.15	12.85	10.49	9.31 ± 1.23	13.21	14.94
	81.00	82.71 ± 11.00	13.30	2.11	77.65 ± 10.42	13.42	−4.14	77.44 ± 10.21	13.18	−4.40
	162.00	159.30 ± 2.85	1.79	−1.67	181.74 ± 15.06	8.29	12.19	164.51 ± 10.80	6.56	1.55