Tissue Distribution of Total Flavonoids Extracts of Drynariae Rhizoma in Young and Old Rats by UPLC–MS/MS Determination

Table 22

Stability of naringenin in rat tissues (n = 6).

Tissues	Spiked (ng/mL)	Room temperature			Freeze-thaw (−20°C, 3 times)			Long term (−20°C, 30 days)
		Mean ± SD	RSD (%)	RE (%)	Mean ± SD	RSD (%)	RE (%)	Mean ± SD	RSD (%)	RE (%)
Heart	8.10	8.04 ± 1.04	12.94	−0.74	8.24 ± 1.17	14.20	1.73	8.00 ± 1.08	13.50	−1.23
	81.00	81.52 ± 11.82	14.50	0.64	84.33 ± 6.52	7.73	4.11	77.37 ± 6.28	8.12	−4.48
	162.00	151.00 ± 20.37	13.49	−6.79	169.65 ± 21.47	12.66	4.72	171.11 ± 25.44	14.87	5.62
Liver	8.10	8.00 ± 0.80	10.00	−1.23	9.27 ± 1.32	14.24	14.44	8.55 ± 1.25	14.62	5.56
	405.00	418.93 ± 47.87	11.43	3.44	436.27 ± 64.51	14.79	7.72	434.87 ± 44.93	10.33	7.38
	810.00	778.74 ± 112.79	14.48	−3.86	771.71 ± 87.80	11.38	−4.73	734.04 ± 37.39	5.09	−9.38
Spleen	8.10	8.58 ± 0.70	8.16	5.93	9.22 ± 0.83	9.00	13.83	8.87 ± 1.19	13.42	9.51
	81.00	80.48 ± 6.41	7.96	−0.64	90.38 ± 0.78	0.86	11.58	83.50 ± 8.26	9.89	3.09
	162.00	139.60 ± 17.83	12.77	−13.83	165.50 ± 7.38	4.46	2.16	156.36 ± 14.57	9.32	−3.48
Lung	8.10	9.15 ± 1.10	12.02	12.96	7.98 ± 0.83	10.40	−1.48	9.31 ± 1.22	13.10	14.98
	81.00	74.57 ± 7.11	9.53	−7.94	84.78 ± 8.89	10.49	4.67	79.09 ± 8.84	11.18	−2.36
	162.00	156.47 ± 22.72	14.52	−3.41	154.67 ± 21.84	14.12	−4.52	167.06 ± 23.04	13.79	3.12
Kidney	8.10	8.45 ± 1.14	13.49	4.32	8.42 ± 1.06	12.59	3.95	8.13 ± 1.09	13.41	0.37
	81.00	77.29 ± 9.32	12.06	−4.58	86.85 ± 6.12	7.05	7.22	83.16 ± 11.44	13.76	2.67
	162.00	167.81 ± 24.47	14.58	3.59	159.11 ± 23.36	14.68	−1.78	162.72 ± 22.74	13.97	0.44
Brain	5.05	4.56 ± 0.49	10.75	−9.70	4.88 ± 0.64	13.11	−3.37	4.71 ± 0.42	8.92	−6.73
	10.10	11.45 ± 1.03	9.00	13.37	10.80 ± 0.98	9.07	6.93	10.81 ± 1.45	13.41	7.03
	25.25	23.97 ± 2.89	12.06	−5.07	28.86 ± 1.83	6.34	14.30	25.78 ± 1.99	7.72	2.10
Stomach	8.10	8.16 ± 0.84	10.29	0.74	8.84 ± 0.84	9.50	9.14	8.35 ± 1.16	13.89	3.09
	405.00	407.95 ± 57.80	14.17	0.73	417.68 ± 62.09	14.87	3.13	435.78 ± 65.10	14.94	7.60
	810.00	843.25 ± 105.05	12.46	4.10	823.51 ± 95.20	11.56	1.67	881.58 ± 126.68	14.37	8.84
Uterus	8.10	9.06 ± 0.83	9.16	11.85	7.79 ± 0.82	10.53	−3.83	8.81 ± 1.28	14.53	8.77
	81.00	73.81 ± 5.18	7.02	−8.88	85.29 ± 11.93	13.99	5.30	73.36 ± 5.25	7.16	−9.43
	162.00	153.95 ± 20.86	13.55	−4.97	172.19 ± 17.57	10.20	6.29	154.24 ± 22.37	14.50	−4.79
Ovary	8.10	7.86 ± 0.67	8.52	−2.96	8.73 ± 1.17	13.40	7.78	7.76 ± 0.50	6.44	−4.20
	81.00	78.53 ± 11.16	14.21	−3.05	86.02 ± 9.25	10.75	6.20	76.30 ± 7.87	10.31	−5.80
	162.00	144.08 ± 10.08	7.00	−11.06	183.49 ± 8.80	4.80	13.27	156.39 ± 23.1	14.77	−3.46
Intestine	8.10	7.69 ± 1.01	13.13	−5.06	7.59 ± 0.54	7.11	−6.30	8.16 ± 0.93	11.40	0.74
	405.00	409.72 ± 54.55	13.31	1.17	399.15 ± 58.51	14.66	−1.44	434.72 ± 60.96	14.02	7.34
	810.00	823.39 ± 83.78	10.18	1.65	717.09 ± 36.44	5.08	−11.47	748.90 ± 78.81	10.52	−7.54