Tissue Distribution of Total Flavonoids Extracts of Drynariae Rhizoma in Young and Old Rats by UPLC–MS/MS Determination

Table 23

Stability of kaempferol in rat tissues (n = 6).

Tissues	Spiked (ng/mL)	Room temperature			Freeze-thaw (−20°C, 3 times)			Long term (−20°C, 30 days)
		Mean ± SD	RSD (%)	RE (%)	Mean ± SD	RSD (%)	RE (%)	Mean ± SD	RSD (%)	RE (%)
Liver	8.10	8.39 ± 1.60	14.04	3.54	8.62 ± 1.09	12.69	6.46	8.57 ± 1.42	14.62	5.76
	40.50	43.77 ± 9.22	13.06	8.08	50.17 ± 2.46	4.90	13.88	46.07 ± 9.62	14.87	13.75
	81.00	83.39 ± 13.38	14.35	2.95	82.41 ± 11.32	13.74	1.74	88.11 ± 13.51	14.33	8.78
Spleen	12.20	11.80 ± 1.91	14.15	−3.28	10.33 ± 0.84	8.09	−14.36	12.19 ± 1.43	11.75	−0.11
	91.50	89.37 ± 5.89	6.59	−2.32	104.78 ± 13.69	13.06	14.52	87.15 ± 2.32	2.66	−4.76
	183.00	189.62 ± 32.89	14.35	3.62	188.06 ± 24.01	12.77	2.77	201.14 ± 33.04	14.43	9.91
Lung	5.05	5.26 ± 0.82	14.68	4.16	4.91 ± 0.45	9.19	−2.84	5.11 ± 0.97	14.94	1.19
	10.10	8.89 ± 0.72	8.05	−11.98	9.79 ± 1.73	14.71	−3.07	9.07 ± 0.74	8.20	−10.23
	25.25	23.20 ± 5.65	14.34	−8.11	24.29 ± 3.55	14.60	−3.79	24.46 ± 6.43	14.31	−3.13
Kidney	12.20	10.56 ± 1.22	11.55	−13.42	10.86 ± 1.26	11.55	−10.96	10.39 ± 1.07	10.34	−14.86
	42.70	41.76 ± 4.97	11.90	−2.20	40.96 ± 5.41	13.21	−4.07	43.77 ± 5.68	12.99	2.51
	85.40	86.58 ± 7.17	8.28	1.39	86.17 ± 11.23	13.04	0.90	91.18 ± 8.09	8.88	6.76
Stomach	5.05	4.77 ± 1.03	11.50	−5.48	4.86 ± 0.38	7.85	−3.83	5.05 ± 0.64	12.74	−0.07
	10.10	11.62 ± 1.09	9.42	14.02	9.41 ± 0.37	3.95	−6.80	10.40 ± 1.12	10.79	2.94
	25.25	24.02 ± 2.30	9.55	−4.87	24.27 ± 4.81	14.82	−3.89	25.50 ± 4.23	14.58	1.00
Uterus	5.05	4.42 ± 0.24	5.35	−12.48	5.37 ± 0.09	1.69	6.27	4.74 ± 0.51	10.80	−6.20
	10.10	11.14 ± 1.60	14.33	10.26	9.57 ± 0.75	7.87	−5.25	10.75 ± 1.57	14.60	6.40
	25.25	22.74 ± 3.64	14.02	−9.95	25.59 ± 0.85	3.31	1.36	25.10 ± 2.16	8.61	−0.58
Intestine	8.10	8.36 ± 1.13	13.49	3.17	8.27 ± 1.20	14.53	2.06	8.54 ± 1.33	14.62	5.43
	121.50	97.69 ± 2.61	2.67	−14.60	112.52 ± 13.53	12.03	−7.39	100.47 ± 2.96	2.95	−14.31
	243.00	241.99 ± 28.92	11.95	−0.42	286.61 ± 39.49	13.78	14.95	265.03 ± 42.79	14.15	9.07