Journal of Analytical Methods in Chemistry

Research Article

Tissue Distribution of Total Flavonoids Extracts of Drynariae Rhizoma in Young and Old Rats by UPLC–MS/MS Determination

Table 24

Tissue concentrations of seven analytes at different times after oral administration in 8-month-old rats (ng/g, n = 5).


Analytes	Tissue	0.25 h	0.5 h	1 h	2 h	4 h	8 h	12 h

Neoeriocitrin	Heart	39.18 ± 10.58	222.17 ± 35.38	173.93 ± 32.14	121.81 ± 30.37	68.13 ± 16.59	9.22 ± 1.68	0.00
	Liver	31.86 ± 7.17	501.02 ± 59.13	3,247.80 ± 413.47	2,201.25 ± 211.27	355.59 ± 119.14	167.65 ± 49.30	14.25 ± 6.75
	Spleen	102.90 ± 13.98	2,190.70 ± 345.97	8,008.91 ± 715.34	1,397.10 ± 286.06	159.13 ± 56.30	36.68 ± 34.51	7.50 ± 1.74
	Lung	1,119.98 ± 146.91	6,039.56 ± 279.40	7,837.99 ± 493.16	5,080.60 ± 431.81	695.30 ± 107.71	34.26 ± 8.21	0.00
	Kidney	134.72 ± 9.14	414.94 ± 60.69	257.50 ± 75.86	112.65 ± 30.13	29.32 ± 9.44	7.65 ± 4.81	0.00
	Brain	13.32 ± 2.84	25.03 ± 2.27	34.04 ± 6.39	8.31 ± 0.92	4.45 ± 1.87	0.00	0.00
	Stomach	4,061.88 ± 312.91	7,324.59 ± 397.44	3,775.38 ± 465.61	1,513.07 ± 264.13	502.61 ± 189.18	25.85 ± 5.70	0.00
	Uterus	75.22 ± 10.79	156.79 ± 16.29	251.62 ± 35.33	70.32 ± 13.94	26.97 ± 4.04	7.26 ± 2.46	0.00
	Ovary	103.83 ± 17.25	162.03 ± 24.58	251.99 ± 32.24	146.23 ± 16.34	12.27 ± 6.13	0.00	0.00
	Intestine	188.58 ± 45.77	4,818.76 ± 299.03	8,133.84 ± 581.81	993.61 ± 114.13	781.08 ± 121.90	163.04 ± 32.57	0.00

Luteolin-7-O-β-D-glucoside	Heart	9.58 ± 2.01	20.71 ± 3.14	15.06 ± 2.10	8.87 ± 2.14	3.27 ± 1.06	0.00	0.00
	Liver	7.44 ± 1.19	21.80 ± 4.17	12.38 ± 2.34	6.65 ± 1.14	3.48 ± 2.53	0.00	0.00
	Spleen	4.11 ± 2.23	7.69 ± 3.58	38.03 ± 7.51	15.62 ± 6.06	7.51 ± 2.77	2.48 ± 1.85	0.00
	Lung	8.82 ± 1.26	24.53 ± 2.02	51.71 ± 3.02	20.08 ± 4.13	7.69 ± 2.51	2.82 ± 3.26	0.00
	Kidney	2.43 ± 1.72	4.81 ± 2.61	8.44 ± 6.55	32.29 ± 9.01	17.40 ± 5.39	2.88 ± 1.44	0.00
	Brain	2.14 ± 0.87	13.17 ± 1.79	7.14 ± 0.92	1.95 ± 0.74	1.05 ± 0.52	0.00	0.00
	Stomach	70.44 ± 7.01	92.66 ± 11.08	64.82 ± 5.00	46.88 ± 9.75	31.71 ± 6.82	3.79 ± 2.90	0.00
	Uterus	8.90 ± 0.95	9.65 ± 0.59	22.33 ± 4.67	6.52 ± 2.08	3.29 ± 2.27	0.00	0.00
	Ovary	6.09 ± 1.20	9.94 ± 3.64	17.25 ± 6.60	20.16 ± 5.62	4.65 ± 2.56	0.00	0.00
	Intestine	21.13 ± 3.16	87.42 ± 11.09	68.78 ± 8.31	40.51 ± 7.63	21.03 ± 7.04	2.71 ± 1.35	0.00

Astragalin	Heart	5.16 ± 2.00	44.98 ± 13.39	21.10 ± 3.34	9.17 ± 1.97	4.55 ± 1.51	1.34 ± 0.67	0.00
	Liver	9.55 ± 1.67	24.38 ± 5.93	63.85 ± 10.04	22.40 ± 3.22	8.48 ± 3.52	4.53 ± 3.04	0.00
	Spleen	4.82 ± 0.94	11.88 ± 2.34	23.04 ± 3.18	41.29 ± 6.27	8.69 ± 4.48	3.32 ± 2.61	0.00
	Lung	9.26 ± 1.67	12.71 ± 1.47	44.28 ± 6.91	31.32 ± 4.41	15.46 ± 5.84	8.06 ± 2.45	2.04 ± 1.02
	Kidney	6.04 ± 1.45	8.64 ± 2.33	13.76 ± 1.92	19.11 ± 1.64	7.87 ± 2.20	2.70 ± 2.12	0.00
	Brain	5.41 ± 1.71	6.08 ± 1.44	27.72 ± 7.78	14.98 ± 1.23	0.13 ± 0.07	0.00	0.00
	Stomach	54.36 ± 13.29	92.09 ± 5.08	124.20 ± 20.11	242.33 ± 28.28	90.80 ± 5.12	12.87 ± 3.39	0.00
	Uterus	9.17 ± 1.60	12.51 ± 1.46	36.06 ± 14.35	21.44 ± 8.70	8.11 ± 1.98	4.13 ± 2.88	0.00
	Ovary	16.09 ± 2.13	41.80 ± 7.89	56.24 ± 5.64	35.79 ± 7.01	6.55 ± 2.67	1.27 ± 0.64	0.00
	Intestine	9.08 ± 2.60	13.45 ± 5.10	46.33 ± 14.85	84.05 ± 6.62	54.11 ± 16.00	0.00	0.00

Naringin	Heart	24.05 ± 3.55	305.07 ± 23.30	802.23 ± 48.96	95.19 ± 19.18	25.10 ± 8.37	18.89 ± 6.74	4.22 ± 3.00
	Liver	3,100.99 ± 329.24	6,844.61 ± 394.09	8,990.54 ± 645.09	4,696.73 ± 787.00	2,309.32 ± 361.78	4,316.78 ± 750.95	87.26 ± 61.30
	Spleen	1,033.31 ± 123.04	2,117.77 ± 261.76	8,347.29 ± 550.68	7,318.88 ± 584.70	4,566.69 ± 415.28	161.26 ± 73.58	65.26 ± 20.78
	Lung	2,403.72 ± 542.57	6,136.89 ± 597.95	8,028.23 ± 577.45	4,758.34 ± 388.04	2,218.44 ± 480.61	261.11 ± 187.44	72.21 ± 44.57
	Kidney	3,898.03 ± 305.12	5,952.40 ± 564.86	6,815.11 ± 427.58	3,831.32 ± 409.31	997.11 ± 113.99	182.31 ± 72.30	10.28 ± 2.11
	Brain	254.15 ± 31.88	914.44 ± 114.98	769.77 ± 106.65	632.54 ± 64.55	403.25 ± 46.45	163.38 ± 36.62	13.74 ± 11.25
	Stomach	1,089.48 ± 158.52	2,523.96 ± 367.74	4,874.73 ± 712.92	6,845.44 ± 660.93	1,957.66 ± 275.96	205.47 ± 86.57	6.92 ± 3.84
	Uterus	1,797.02 ± 178.68	3,515.04 ± 532.80	4,022.84 ± 195.00	3,052.92 ± 310.56	1,130.50 ± 192.81	2,476.23 ± 917.77	38.34 ± 29.09
	Ovary	3,884.52 ± 446.17	5,049.08 ± 241.23	8,319.83 ± 564.53	2,283.59 ± 417.38	1,434.68 ± 357.23	113.37 ± 34.10	0.00
	Intestine	1,192.96 ± 191.76	4,933.79 ± 803.89	8,510.36 ± 952.27	11,753.34 ± 1,203.70	1,465.91 ± 469.36	448.75 ± 196.23	0.00

Eriodictyol	Liver	19.37 ± 2.97	23.76 ± 2.79	144.70 ± 20.72	71.92 ± 16.12	136.87 ± 30.56	96.07 ± 48.74	0.00
	Spleen	0.00	4.29 ± 1.55	19.26 ± 1.84	26.20 ± 4.64	2.11 ± 0.23	0.00	0.00
	Lung	49.46 ± 6.08	30.50 ± 7.01	19.56 ± 2.94	8.70 ± 1.76	3.87 ± 2.91	0.00	0.00
	Stomach	58.57 ± 7.99	89.49 ± 17.62	105.23 ± 20.57	148.36 ± 15.79	80.59 ± 7.28	13.04 ± 4.34	0.00
	Uterus	8.46 ± 1.82	16.52 ± 4.19	33.10 ± 4.66	8.25 ± 5.11	1.08 ± 0.06	0.00	0.00
	Ovary	6.21 ± 0.72	10.50 ± 1.41	32.09 ± 2.87	10.05 ± 1.43	4.08 ± 1.95	0.00	0.00
	Intestine	37.69 ± 12.93	138.00 ± 61.75	209.76 ± 71.61	69.79 ± 20.12	26.10 ± 9.96	6.25 ± 1.06	0.00

Naringenin	Heart	2.45 ± 0.78	5.15 ± 1.12	7.68 ± 1.07	21.54 ± 3.80	29.00 ± 5.82	182.90 ± 11.46	0.00
	Liver	17.88 ± 2.93	31.73 ± 6.68	46.63 ± 5.29	155.17 ± 25.16	761.78 ± 99.66	837.21 ± 69.13	23.80 ± 12.62
	Spleen	11.50 ± 1.36	16.51 ± 3.35	30.39 ± 11.29	165.29 ± 11.28	97.20 ± 18.16	57.48 ± 13.95	11.16 ± 9.47
	Lung	11.66 ± 1.74	37.15 ± 6.87	63.24 ± 4.93	53.05 ± 7.00	48.79 ± 12.05	101.33 ± 17.69	3.31 ± 0.63
	Kidney	8.65 ± 2.02	39.65 ± 4.97	54.99 ± 7.07	48.80 ± 7.84	53.29 ± 8.58	103.53 ± 14.66	0.00
	Brain	3.27 ± 1.49	9.39 ± 3.66	5.01 ± 1.91	0.80 ± 0.61	0.00	0.00	0.00
	Stomach	234.16 ± 20.86	313.03 ± 55.49	369.35 ± 40.89	622.55 ± 25.86	435.45 ± 91.57	162.98 ± 18.45	8.57 ± 1.15
	Uterus	17.51 ± 3.47	24.28 ± 4.26	141.20 ± 18.48	79.43 ± 14.57	182.01 ± 20.39	118.06 ± 33.90	8.92 ± 1.85
	Ovary	16.78 ± 5.55	28.05 ± 7.04	132.19 ± 34.53	75.75 ± 9.82	185.35 ± 10.12	137.40 ± 23.25	0.00
	Intestine	13.35 ± 2.46	31.49 ± 14.73	140.07 ± 31.41	60.03 ± 15.27	144.57 ± 40.01	98.08 ± 45.70	0.00

Kaempferol	Liver	0.67 ± 0.35	11.53 ± 4.46	68.82 ± 13.01	49.80 ± 14.43	17.44 ± 5.63	2.51 ± 1.60	0.00
	Spleen	0.00	9.67 ± 3.02	182.98 ± 17.07	22.46 ± 3.82	16.06 ± 6.56	8.96 ± 7.29	0.00
	Lung	11.40 ± 1.07	24.08 ± 2.27	29.52 ± 1.85	19.73 ± 1.14	8.78 ± 2.16	0.87 ± 0.74	0.00
	Kidney	16.19 ± 3.45	24.34 ± 5.27	31.90 ± 5.32	55.28 ± 5.99	22.71 ± 4.80	0.00	0.00
	Stomach	17.25 ± 1.93	24.54 ± 2.69	39.83 ± 13.60	43.81 ± 5.36	21.79 ± 7.01	4.95 ± 3.58	0.00
	Uterus	3.36 ± 1.70	6.19 ± 0.85	24.01 ± 3.45	18.35 ± 3.34	12.50 ± 1.60	0.00	0.00
	Intestine	44.75 ± 8.17	80.12 ± 14.32	405.27 ± 37.64	348.26 ± 12.33	310.25 ± 42.39	39.37 ± 8.12	0.00