Journal of Analytical Methods in Chemistry

Research Article

Tissue Distribution of Total Flavonoids Extracts of Drynariae Rhizoma in Young and Old Rats by UPLC–MS/MS Determination

Table 25

Tissue concentrations of seven analytes at different times after oral administration in 18-month-old rats (ng/g, n = 5).


Analytes	Tissue	0.25 h	0.5 h	1 h	2 h	4 h	8 h	12 h

Neoeriocitrin	Heart	31.73 ± 3.60	189.07 ± 21.88	164.81 ± 17.36	101.18 ± 12.35	77.21 ± 5.02	25.17 ± 2.90	0.00
	Liver	16.96 ± 1.53	401.00 ± 31.39	2,431.18 ± 217.03	1,571.02 ± 164.80	677.16 ± 48.76	305.28 ± 35.76	0.00
	Spleen	230.11 ± 15.95	2,006.98 ± 117.28	5,970.17 ± 737.71	2,543.47 ± 175.93	1,114.32 ± 88.74	377.12 ± 35.87	0.00
	Lung	1,927.71 ± 172.26	5,092.49 ± 130.22	6,223.83 ± 597.97	4,985.02 ± 490.80	1,463.71 ± 179.74	372.35 ± 38.97	0.00
	Kidney	97.28 ± 8.59	387.12 ± 44.81	235.05 ± 27.59	127.05 ± 7.19	66.06 ± 8.01	13.80 ± 1.59	0.00
	Brain	8.15 ± 0.75	20.59 ± 1.42	24.98 ± 1.99	7.41 ± 0.70	6.47 ± 0.47	0.00	0.00
	Stomach	3,632.23 ± 336.19	5,552.27 ± 384.05	3,964.81 ± 315.74	2,299.33 ± 278.83	1,039.50 ± 119.57	585.36 ± 46.62	68.88 ± 6.55
	Uterus	49.22 ± 4.45	106.19 ± 8.31	180.98 ± 16.16	78.08 ± 8.19	40.89 ± 2.94	17.19 ± 2.02	0.00
	Ovary	78.22 ± 4.57	111.62 ± 10.33	170.29 ± 11.78	138.69 ± 11.04	64.70 ± 6.15	41.51 ± 3.00	0.00
	Intestine	1,646.14 ± 96.19	4,254.15 ± 393.76	6,236.26 ± 431.37	3,118.61 ± 248.35	1,426.29 ± 102.70	463.22 ± 54.26	0.00

Luteolin- 7-O-β-D-glucoside	Heart	10.88 ± 1.03	49.05 ± 4.38	29.95 ± 0.77	12.27 ± 1.17	6.94 ± 0.68	6.38 ± 0.78	0.00
	Liver	6.98 ± 0.48	21.34 ± 1.25	48.17 ± 4.46	27.24 ± 1.18	16.49 ± 1.31	9.14 ± 2.54	3.79 ± 3.17
	Spleen	4.95 ± 0.36	12.27 ± 0.69	22.73 ± 2.76	38.76 ± 4.46	14.50 ± 1.15	5.44 ± 0.52	0.00
	Lung	8.68 ± 0.64	11.67 ± 0.66	24.51 ± 2.97	34.10 ± 3.92	13.35 ± 1.63	7.91 ± 0.51	0.00
	Kidney	4.68 ± 0.37	6.35 ± 0.57	11.03 ± 1.16	15.63 ± 1.08	9.87 ± 0.79	6.05 ± 0.58	0.00
	Brain	3.80 ± 0.34	9.14 ± 1.06	25.02 ± 2.94	12.37 ± 0.95	5.11 ± 0.57	0.00	0.00
	Stomach	39.57 ± 4.58	75.86 ± 8.90	130.26 ± 10.04	205.52 ± 22.86	136.00 ± 13.96	26.54 ± 3.51	14.21 ± 1.59
	Uterus	7.72 ± 0.74	10.10 ± 0.99	24.21 ± 2.97	19.84 ± 1.58	12.60 ± 1.20	6.98 ± 0.51	0.00
	Ovary	14.85 ± 1.16	41.55 ± 3.71	38.08 ± 3.99	30.21 ± 3.97	11.18 ± 1.15	0.00	0.00
	Intestine	7.72 ± 0.69	8.97 ± 0.23	38.79 ± 3.71	701.13 ± 6.90	48.59 ± 5.89	14.75 ± 1.70	0.00

Astragalin	Heart	6.39 ± 0.67	21.86 ± 2.21	13.27 ± 1.19	10.78 ± 0.84	5.80 ± 0.52	4.42 ± 0.50	0.00
	Liver	6.93 ± 0.61	18.64 ± 2.16	14.38 ± 1.69	9.51 ± 0.73	7.18 ± 0.80	5.31 ± 1.13	0.00
	Spleen	2.77 ± 0.16	4.42 ± 0.41	30.81 ± 2.13	17.57 ± 1.40	9.09 ± 0.86	6.04 ± 0.44	0.00
	Lung	7.85 ± 0.79	20.13 ± 1.81	39.92 ± 3.12	17.98 ± 1.62	10.30 ± 1.16	7.32 ± 0.82	0.00
	Kidney	2.45 ± 0.17	3.57 ± 0.21	10.31 ± 0.95	34.03 ± 2.35	16.68 ± 1.33	8.76 ± 0.83	3.41 ± 0.25
	Brain	3.23 ± 0.37	10.09 ± 1.17	7.11 ± 0.75	3.84 ± 0.47	2.14 ± 0.14	0.00	0.00
	Stomach	50.47 ± 5.31	73.23 ± 9.63	124.20 ± 20.11	242.33 ± 28.28	90.80 ± 5.12	12.87 ± 3.39	0.00
	Uterus	7.05 ± 0.74	8.07 ± 1.06	15.35 ± 1.58	10.07 ± 1.33	6.87 ± 0.62	4.08 ± 0.46	0.00
	Ovary	5.59 ± 0.59	9.39 ± 1.23	14.14 ± 1.45	17.47 ± 1.79	9.13 ± 1.21	4.01 ± 0.45	0.00
	Intestine	17.15 ± 1.00	73.74 ± 6.83	53.74 ± 3.72	35.12 ± 4.04	15.60 ± 1.24	7.20 ± 0.68	0.00

Naringin	Heart	15.87 ± 2.49	201.48 ± 20.58	643.80 ± 91.40	99.05 ± 15.19	40.88 ± 2.88	22.64 ± 2.88	10.91 ± 1.45
	Liver	2,848.51 ± 428.98	4,590.53 ± 744.43	7,219.96 ± 1,059.27	3,393.97 ± 490.96	1,933.20 ± 259.73	4,047.07 ± 309.31	90.95 ± 10.55
	Spleen	827.46 ± 77.63	1,997.58 ± 151.10	7,005.53 ± 555.82	4,646.08 ± 509.30	3,556.25 ± 666.72	1,077.91 ± 166.30	187.62 ± 28.35
	Lung	2,467.96 ± 219.09	4,314.82 ± 373.65	5,985.15 ± 291.65	3,011.26 ± 247.62	2,118.82 ± 171.12	280.11 ± 33.42	83.92 ± 10.13
	Kidney	3,004.22 ± 241.98	5,728.09 ± 367.43	7,354.37 ± 678.80	3,414.98 ± 295.76	1,578.24 ± 75.40	621.79 ± 17.60	130.97 ± 3.45
	Brain	180.55 ± 11.86	844.75 ± 43.48	756.89 ± 133.87	500.91 ± 16.24	371.38 ± 20.03	127.58 ± 7.22	42.62 ± 2.27
	Stomach	934.78 ± 63.59	2,648.34 ± 29.62	11,647.56 ± 856.48	5,195.02 ± 398.77	2,449.35 ± 120.89	266.57 ± 18.89	8.42 ± 0.65
	Uterus	1,367.90 ± 24.22	3,012.12 ± 11.46	3,367.69 ± 178.65	2,330.25 ± 166.02	1,300.67 ± 153.39	2,066.60 ± 132.32	71.21 ± 3.31
	Ovary	3,842.79 ± 127.02	4,546.92 ± 322.21	6,362.25 ± 267.28	17,813.64 ± 785.27	17,192.38 ± 9,967.81	363.64 ± 29.11	31.74 ± 2.55
	Intestine	673.87 ± 58.98	3,654.42 ± 267.86	6,084.22 ± 648.83	9,323.07 ± 754.96	2,177.045 ± 128.14	965.96 ± 24.06	66.80 ± 4.91

Eriodictyol	Liver	16.96 ± 1.61	30.10 ± 4.27	36.17 ± 5.19	13.66 ± 1.89	8.19 ± 0.65	3.16 ± 2.73	0.00
	Spleen	0.00	11.15 ± 1.26	25.17 ± 2.83	39.09 ± 2.48	0.00	0.00	0.00
	Lung	47.00 ± 3.08	20.90 ± 3.85	15.87 ± 2.04	7.50 ± 0.47	5.48 ± 0.81	0.00	0.00
	Stomach	57.23 ± 5.34	64.35 ± 9.44	70.60 ± 10.74	146.73 ± 19.87	66.77 ± 8.91	11.51 ± 1.30	0.00
	Uterus	8.60 ± 0.74	9.54 ± 0.88	35.62 ± 2.90	8.08 ± 1.13	0.00	0.00	0.00
	Ovary	5.97 ± 0.80	8.92 ± 0.63	24.97 ± 2.10	8.80 ± 1.10	6.30 ± 0.98	0.00	0.00
	Intestine	21.73 ± 2.65	83.46 ± 11.14	130.28 ± 19.34	43.59 ± 3.71	13.44 ± 1.34	8.58 ± 1.31	0.00

Naringenin	Heart	3.23 ± 0.34	8.60 ± 0.87	10.44 ± 0.94	18.90 ± 1.48	37.96 ± 3.42	95.36 ± 10.72	14.82 ± 1.66
	Liver	14.98 ± 1.42	26.78 ± 2.39	40.47 ± 1.03	121.58 ± 11.64	616.85 ± 60.73	413.12 ± 50.73	15.99 ± 1.67
	Spleen	8.09 ± 1.07	12.22 ± 2.12	15.01 ± 2.10	83.01 ± 10.49	69.71 ± 6.26	111.57 ± 13.09	15.14 ± 8.78
	Lung	7.67 ± 2.00	26.33 ± 7.58	37.52 ± 9.28	54.69 ± 5.73	61.63 ± 4.50	94.13 ± 9.13	10.88 ± 5.77
	Kidney	2.91 ± 0.35	8.62 ± 0.78	54.76 ± 4.29	41.12 ± 3.67	47.99 ± 5.03	175.34 ± 12.62	5.08 ± 0.59
	Brain	3.96 ± 0.40	9.95 ± 1.05	5.91 ± 0.78	13.22 ± 1.36	11.52 ± 1.53	18.79 ± 2.11	0.00
	Stomach	136.19 ± 16.40	164.32 ± 14.87	255.32 ± 19.99	393.85 ± 35.16	323.57 ± 33.94	210.95 ± 15.19	0.00
	Uterus	10.67 ± 1.07	17.14 ± 1.80	99.58 ± 13.09	47.72 ± 4.90	120.04 ± 15.89	100.46 ± 11.27	0.00
	Ovary	6.55 ± 0.45	9.46 ± 0.55	19.22 ± 1.78	58.95 ± 4.08	67.62 ± 5.38	87.30 ± 8.30	0.00
	Intestine	57.06 ± 4.18	84.62 ± 4.79	142.69 ± 17.30	391.10 ± 44.99	565.62 ± 45.04	499.71 ± 47.52	0.00

Kaempferol	Liver	0.00	0.00	7.75 ± 1.12	37.65 ± 2.13	43.61 ± 5.29	10.18 ± 1.17	0.00
	Spleen	0.00	13.30 ± 1.51	199.66 ± 23.11	25.68 ± 2.71	24.49 ± 2.99	16.70 ± 1.08	0.00
	Lung	12.21 ± 1.28	22.19 ± 2.73	32.75 ± 4.10	15.20 ± 1.15	5.19 ± 0.81	3.52 ± 1.14	0.00
	Kidney	0.66 ± 0.06	20.79 ± 2.41	38.34 ± 4.51	67.31 ± 5.19	29.74 ± 3.31	0.00	0.00
	Stomach	7.46 ± 0.75	20.55 ± 2.16	24.88 ± 3.27	34.72 ± 3.56	17.44 ± 2.31	2.73 ± 0.31	0.00
	Uterus	3.03 ± 0.27	6.23 ± 0.72	21.54 ± 2.53	15.86 ± 1.22	11.30 ± 1.26	5.64 ± 0.63	0.00
	Intestine	25.24 ± 1.48	54.96 ± 5.09	159.55 ± 11.04	120.50 ± 9.60	98.37 ± 9.36	46.17 ± 3.34	0.00