Tissue Distribution of Total Flavonoids Extracts of Drynariae Rhizoma in Young and Old Rats by UPLC–MS/MS Determination

Table 4

Interday and intraday precision and accuracy data for assays of luteolin-7-O-β-D-glucoside in different tissues (n = 6).

Tissues	Spiked (ng/mL)	Interday			Intraday
		Mean ± SD	Precision (RSD, %)	Relative error (RE, %)	Mean ± SD	Precision (RSD, %)	Relative error (RE, %)
Heart	5.05	5.09 ± 0.56	11.00	0.79	5.22 ± 0.64	12.26	3.37
	10.10	9.74 ± 1.30	13.35	−3.56	10.28 ± 1.15	11.19	1.78
	25.25	23.44 ± 2.76	11.77	−7.17	22.49 ± 2.68	11.92	−10.93
Liver	5.05	4.81 ± 0.69	14.35	−4.75	4.95 ± 0.70	14.14	−1.98
	10.10	9.23 ± 0.98	10.62	−8.61	9.12 ± 1.17	12.83	−9.70
	25.25	24.52 ± 2.55	10.40	−2.89	25.12 ± 1.67	6.65	−0.51
Spleen	5.05	4.70 ± 0.62	13.19	−6.93	4.86 ± 0.70	14.40	−3.76
	10.10	10.67 ± 1.51	14.15	5.64	11.05 ± 1.45	13.12	9.41
	25.25	25.98 ± 1.62	6.24	2.89	25.97 ± 1.08	4.16	2.85
Lung	8.10	8.61 ± 0.64	7.43	6.30	8.87 ± 0.64	7.22	9.51
	40.50	39.29 ± 5.51	14.02	−2.99	36.05 ± 2.02	5.60	−10.99
	81.00	82.44 ± 3.24	3.93	1.78	82.32 ± 3.53	4.29	1.63
Kidney	5.05	4.90 ± 0.62	12.65	−2.97	4.78 ± 0.56	11.72	−5.35
	10.10	10.43 ± 1.50	14.38	3.27	10.70 ± 1.45	13.55	5.94
	25.25	25.07 ± 2.34	9.33	−0.71	24.93 ± 2.90	11.63	−1.27
Brain	5.05	4.82 ± 0.43	8.92	−4.55	4.63 ± 0.41	8.86	−8.32
	10.10	10.40 ± 1.48	14.23	2.97	10.36 ± 1.09	10.52	2.57
	25.25	24.69 ± 3.04	12.31	−2.22	25.89 ± 3.01	11.63	2.53
Stomach	8.10	7.94 ± 0.76	9.57	−1.98	7.55 ± 0.28	3.71	−6.79
	40.50	40.41 ± 4.15	10.27	−0.22	39.04 ± 2.31	5.92	−3.60
	81.00	81.58 ± 5.19	6.36	0.72	83.26 ± 5.63	6.76	2.79
Uterus	5.05	4.60 ± 0.61	13.26	−8.91	4.57 ± 0.54	11.82	−9.50
	10.10	8.99 ± 0.43	4.78	−10.99	9.08 ± 0.52	5.73	−10.10
	25.25	24.19 ± 2.98	12.32	−4.20	23.33 ± 2.55	10.93	−7.60
Ovary	5.05	4.57 ± 0.63	13.79	−9.50	4.74 ± 0.71	14.98	−6.14
	10.10	9.61 ± 1.37	14.26	−4.85	9.90 ± 1.37	13.84	−1.98
	25.25	24.48 ± 1.22	4.98	−3.05	23.78 ± 0.63	2.65	−5.82
Intestine	8.10	8.19 ± 1.02	12.45	1.11	7.63 ± 0.65	8.52	−5.80
	40.50	39.08 ± 5.23	13.38	−3.51	37.96 ± 3.89	10.25	−6.27
	81.00	82.05 ± 9.79	11.93	1.30	78.50 ± 10.40	13.25	−3.09