Research Article
Tissue Distribution of Total Flavonoids Extracts of Drynariae Rhizoma in Young and Old Rats by UPLC–MS/MS Determination
Table 9
Interday and intraday precision and accuracy data for assays of kaempferol in different tissues (n = 6).
| Tissues | Spiked (ng/mL) | Interday | Intraday | Mean ± SD | Precision (RSD, %) | Relative error (RE, %) | Mean ± SD | Precision (RSD, %) | Relative error (RE, %) |
| Liver | 8.10 | 8.50 ± 1.23 | 14.47 | 4.94 | 8.36 ± 1.19 | 14.23 | 3.21 | 40.50 | 45.97 ± 6.78 | 14.75 | 13.51 | 45.61 ± 5.04 | 11.05 | 12.62 | 81.00 | 82.90 ± 11.10 | 13.39 | 2.35 | 85.23 ± 12.01 | 14.09 | 5.22 |
| Spleen | 12.20 | 11.06 ± 1.54 | 13.92 | −9.34 | 10.75 ± 0.72 | 6.70 | −11.89 | 91.50 | 97.07 ± 12.65 | 13.03 | 6.09 | 94.94 ± 10.93 | 11.51 | 3.76 | 183.00 | 188.84 ± 25.77 | 13.65 | 3.19 | 180.04 ± 22.90 | 12.72 | −1.62 |
| Lung | 5.05 | 5.08 ± 0.63 | 12.40 | 0.59 | 4.76 ± 0.30 | 6.30 | −5.74 | 10.10 | 9.34 ± 1.28 | 13.70 | −7.52 | 8.67 ± 0.50 | 5.77 | −14.16 | 25.25 | 23.74 ± 3.26 | 13.73 | −5.98 | 22.77 ± 3.05 | 13.39 | −9.82 |
| Kidney | 12.20 | 10.71 ± 1.12 | 10.46 | −12.21 | 11.37 ± 0.55 | 4.84 | −6.80 | 42.70 | 41.36 ± 4.67 | 11.29 | −3.14 | 43.56 ± 4.11 | 9.44 | 2.01 | 85.40 | 86.37 ± 8.43 | 9.76 | 1.14 | 90.48 ± 6.45 | 7.13 | 5.95 |
| Stomach | 5.05 | 4.81 ± 0.69 | 14.35 | −4.75 | 4.82 ± 0.59 | 12.24 | −4.55 | 10.10 | 10.51 ± 1.41 | 13.42 | 4.06 | 10.64 ± 1.47 | 13.82 | 5.35 | 25.25 | 24.14 ± 3.37 | 13.96 | −4.40 | 25.50 ± 3.41 | 13.37 | 0.99 |
| Uterus | 5.05 | 4.89 ± 0.54 | 11.04 | −3.17 | 4.91 ± 0.50 | 10.18 | −2.77 | 10.10 | 10.35 ± 1.41 | 13.62 | 2.48 | 10.16 ± 1.10 | 10.83 | 0.59 | 25.25 | 24.16 ± 2.84 | 11.75 | −4.32 | 23.47 ± 3.31 | 14.10 | −7.05 |
| Intestine | 8.10 | 8.31 ± 1.04 | 12.52 | 2.59 | 8.32 ± 1.15 | 13.82 | 2.72 | 121.50 | 105.10 ± 11.92 | 11.34 | −13.50 | 104.54 ± 5.05 | 4.83 | −13.96 | 243.00 | 264.29 ± 39.44 | 14.92 | 8.76 | 271.43 ± 36.43 | 13.42 | 11.70 |
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