Research Article

Tissue Distribution of Total Flavonoids Extracts of Drynariae Rhizoma in Young and Old Rats by UPLC–MS/MS Determination

Table 9

Interday and intraday precision and accuracy data for assays of kaempferol in different tissues (n = 6).

TissuesSpiked (ng/mL)InterdayIntraday
Mean ± SDPrecision (RSD, %)Relative error (RE, %)Mean ± SDPrecision (RSD, %)Relative error (RE, %)

Liver8.108.50 ± 1.2314.474.948.36 ± 1.1914.233.21
40.5045.97 ± 6.7814.7513.5145.61 ± 5.0411.0512.62
81.0082.90 ± 11.1013.392.3585.23 ± 12.0114.095.22

Spleen12.2011.06 ± 1.5413.92−9.3410.75 ± 0.726.70−11.89
91.5097.07 ± 12.6513.036.0994.94 ± 10.9311.513.76
183.00188.84 ± 25.7713.653.19180.04 ± 22.9012.72−1.62

Lung5.055.08 ± 0.6312.400.594.76 ± 0.306.30−5.74
10.109.34 ± 1.2813.70−7.528.67 ± 0.505.77−14.16
25.2523.74 ± 3.2613.73−5.9822.77 ± 3.0513.39−9.82

Kidney12.2010.71 ± 1.1210.46−12.2111.37 ± 0.554.84−6.80
42.7041.36 ± 4.6711.29−3.1443.56 ± 4.119.442.01
85.4086.37 ± 8.439.761.1490.48 ± 6.457.135.95

Stomach5.054.81 ± 0.6914.35−4.754.82 ± 0.5912.24−4.55
10.1010.51 ± 1.4113.424.0610.64 ± 1.4713.825.35
25.2524.14 ± 3.3713.96−4.4025.50 ± 3.4113.370.99

Uterus5.054.89 ± 0.5411.04−3.174.91 ± 0.5010.18−2.77
10.1010.35 ± 1.4113.622.4810.16 ± 1.1010.830.59
25.2524.16 ± 2.8411.75−4.3223.47 ± 3.3114.10−7.05

Intestine8.108.31 ± 1.0412.522.598.32 ± 1.1513.822.72
121.50105.10 ± 11.9211.34−13.50104.54 ± 5.054.83−13.96
243.00264.29 ± 39.4414.928.76271.43 ± 36.4313.4211.70