Development of a Chemiluminescence Assay for Total N-Terminal Propeptide of Type I Collagen and Its Evaluation in Lung Transplantation

Scheme 1

Schematic representation of P1NP chemiluminescence assay. The assay uses one-step sandwich format for P1NP quantitative measurement of P1NP: 20 μL sample was added into 80 μL labeling antibody solutions and followed by adding 80 μL biotinylated antibody solutions; after incubation for 30 min at 37°C, 30 L streptavidin beads were added into complex. The immune complex was then separated and washed 3 times. Substrate was added to read the RLU value. The RLU value represent the concentration of P1NP in sample.