Journal of Analytical Methods in Chemistry / 2022 / Article / Tab 1 / Research Article
Development and Validation of Ultrahigh-Performance Liquid Chromatography Coupled with Triple Quadrupole Mass Spectrometry Method for Quantitative Determination of Ten Active Compounds in Ge-Gen-Jiao-Tai-Wan Table 1 Analytical MRM parameters of the developed UHPLC-QQQ-MS method.
Analyte Precursor ion Product ion Fragmentor (V) CE (V) Polarity Quantifier/qualifier Puerarin 417.1 297.1 90 32 Positive Quantifier 417.1 267.0 90 32 Positive Qulifier Daidzin 417.1 255.0 90 15 Positive Quantifier 417.1 199.0 90 50 Positive Qulifier Coptisine 320.1 292.0 110 30 Positive Quantifier 320.1 277.0 110 40 Positive Qulifier Epiberberine 335.9 319.9 110 32 Positive Quantifier 335.9 291.9 110 35 Positive Qulifier Jatrorrhizine 338.1 322.0 120 32 Positive Quantifier 338.1 294.1 120 28 Positive Qulifier Berberine 335.9 319.9 110 32 Positive Quantifier 335.9 291.9 110 35 Positive Qulifier Palmatine 352.1 336.1 85 30 Positive Quantifier 352.1 308.1 85 28 Positive Qulifier Coumarin 146.9 91.1 80 25 Positive Quantifier 146.9 103.0 80 15 Positive Qulifier Daidzein 254.9 91.1 100 40 Positive Quantifier 254.9 199.0 100 25 Positive Qulifier Cinnamic acid 146.8 103.0 80 7 Negative Quantifier
