Development and Validation of Ultrahigh-Performance Liquid Chromatography Coupled with Triple Quadrupole Mass Spectrometry Method for Quantitative Determination of Ten Active Compounds in Ge-Gen-Jiao-Tai-Wan
Table 2
Regression equation, correlation coefficient, linear range, LOD, and LOQ of 10 components in UHPLC-QQQ-MS.
Analyte
Regression equation
Correlation Coefficient (R2)
Linear range (µg/mL)
LOD (ng/mL)
LOQ (ng/mL)
Puerarin
y = 181790.01 x + 29.65
0.9995
0.00774～2.048
1.12
3.93
Daidzin
y = 703318.66 x + 481.60
0.9992
0.00826～1.918
0.88
3.01
Coptisine
y = 926264.11 x– 1293.07
0.9998
0.00444～0.894
0.75
2.32
Epiberberine
y = 2870685.59 x + 965.63
0.9996
0.00174～0.471
0.25
0.86
Jatrorrhizine
y = 2719691.61 x– 352.51
0.9995
0.00323～0.906
0.50
1.73
Berberine
y= 2845616.29x – 1165.64
0.9994
0.00175～0.4775
0.31
1.15
Palmatine
y = 3200432.28 x– 521.16
0.9994
0.00175～0.487
0.28
0.90
Coumarin
y = 251481.47 x– 53.44
0.9998
0.00283～0.734
0.43
1.44
Daidzein
y = 120736.64 x + 16.89
0.9995
0.00112～0.3055
0.17
0.63
Cinnamic acid
y = 6148.94 x– 6.12
0.9996
0.01633～3.695
2.09
6.46
y: peak area of the components; x: concentration in µg/mL.
