A Human Oral Fluid Assay for D- and L- Isomer Detection of Amphetamine and Methamphetamine Using Liquid-Liquid Extraction

Table 1

LC-MS/MS conditions for the three assays on a single oral fluid sample.

	D- and L-
Scan type	MRM
Ion source	Turbo spray
Probe position	X = 5.00, Y = 5.2
Polarity	Positive
Run duration	11 min
Settling time (msec)	0
Pause time (msec)	7.007 msec
Curtain gas	35
CAD gas	4
ISV (V)	5000
Temperature (°C)	500
Ion source gas 1 (GS 1)	50
Ion source gas 2 (GS 2)	50
Q1/Q3 resolution:	unit/unit
CEM (V)	2600
Inlet settings
Analytical column	Supelco Astek Chirobiotic V 250 × 2.1 mm, 5 μm
Guard cartridge	None
Sample temperature	15 ± 5.0°C
Column temperature	30.0 ± 5.0°C
Mobile phase A	Water : acetic acid : ammonium hydroxide : Methanol 5 : 1 : 0.3 : 993.5
Mobile phase B	N/A
Needle rinse	Water : acetic acid : ammonium hydroxide : Methanol 5 : 1 : 0.3 : 993.5
Flow rate	0.3 mL/min
Injection volume	10 μL
Run time	11 min

Note. collision gas (CAD), ion source voltage (ISV), channel electron multiplier (CEM).