Pharmacokinetic Analysis of Diosgenin in Rat Plasma by a UPLC-MS/MS Approach

Table 3

Stability of diosgenin for the determination of the three concentrations in rat plasma (n = 6).

	Concentration (ng/ml)		RSD/%
	Indicated	Measured
Short-term stability at 25°C for 8 h	5	5.41 ± 0.33	6.09
	50	50.23 ± 2.56	5.10
	1000	1000.88 ± 44.34	4.43
Long-term stability at −20°C for 30 days	5	5.99 ± 0.28	4.67
	50	50.02 ± 3.72	7.43
	1000	1001.65 ± 42.42	4.23
Freeze-thaw stability, three cycles	5	5.98 ± 0.35	5.85
	50	51.69 ± 4.36	8.43
	1000	1002.9 ± 54.44	5.42
Autosampler stability at 4°C for 24 h	5	5.32 ± 0.37	6.95
	50	50.59 ± 3.69	5.42
	1000	1001.01 ± 62.63	5.42