LC-MS/MS Method for Determination of Hydroxychloroquine and Metabolites: Application in a Pharmacokinetic Study

Table 4

Interday and intraday precision and accuracy of four analytes in rat blood (n = 5).

Analytes	Nominal concentration (ng/mL)	Intraday (n = 5)			Interday (n = 5)
		Measured concentration (mean ± SD, ng/mL)	Precision (RSD%)	Accuracy (RE%)	Measured concentration (mean ± SD, ng/mL)	Precision (RSD%)	Accuracy (RE%)
BDCQ	1	1.03 ± 0.005	0.56	3.98	1.02 ± 0.02	2.59	2.45
	2.5	2.65 ± 0.05	1.99	6.34	2.54 ± 0.15	6.24	1.82
	1000	1085.1 ± 14.4	1.33	8.51	1097.3 ± 40.1	3.66	9.73
	2000	1797.67 ± 91.65	5.10	−10.11	1758.68 ± 59.67	3.39	−12.06
DCQ	1	1.04 ± 0.02	2.43	4.26	1.03 ± 0.04	4.15	3.49
	2.5	2.57 ± 0.07	2.80	3.19	2.55 ± 0.08	3.24	2.23
	1000	1057.50 ± 86.3	8.17	5.75	1044.80 ± 81.4	7.80	4.48
	2000	2029.26 ± 157.29	7.75	1.46	2033.73 ± 138.43	6.81	1.68
DHCQ	1	1.04 ± 0.03	3.37	4.83	1.02 ± 0.03	3.45	2.39
	2.5	2.61 ± 0.09	3.77	4.67	2.53 ± 0.20	8.15	1.34
	1000	1096.61 ± 56.7	5.18	6.69	1097.0 ± 58.9	5.37	9.70
	2000	1815.69 ± 23.66	1.30	−9.21	1796.74 ± 33.65	1.87	−10.16
HCQ	2	2.02 ± 0.02	1.21	1.20	2.06 ± 0.06	3.13	3.02
	5	5.14 ± 0.10	2.11	2.94	5.05 ± 0.20	4.14	1.11
	2000	2040.6 ± 175.1	8.59	2.04	2022.1 ± 158.1	7.82	1.10
	4000	4276.65 ± 368.71	8.62	6.91	4262.63 ± 321.74	7.55	6.56