Simultaneous Determination of 32 Pyrrolizidine Alkaloids in Two Traditional Chinese Medicine Preparations by UPLC-MS/MS

Table 1

Retention time, concentration, and mass spectrometry conditions for 32 PAs.

Compound^1	Retention time^2 (min)	Concentration (μg/mL)	Quantitative transition			Qualitative transition
			Precursor ion	Product ion	CE^3	Precursor ion	Product ion	CE
Em	13.04	0.3926	398.1	120.1	32	398.1	149.0	30
Er	2.90	1.0325	350.1	120.1	37	350.1	138.1	37
Eu	3.94	0.4048	330.1	138.1	30	330.1	156.2	40
He	7.56	0.4088	314.2	138.1	30	314.2	156.2	35
Ic	4.13	0.3958	300.2	156.2	40	300.2	138.1	30
Im	3.89	0.4128	300.2	156.2	40	300.2	138.1	30
Jb	3.68	1.0135	352.2	120.0	35	352.2	155.1	38
Lc	13.56	0.4038	412.1	120.1	35	412.1	138.1	40
Ly	4.13	0.4118	300.2	138.1	10	300.2	156.2	10
Mc	2.21	1.0300	326.0	138.1	40	326.0	120.1	45
Re	6.50	2.0300	352.2	120.1	40	352.2	138.1	40
Sk	13.50	1.0045	366.1	168.0	9	366.1	150.0	9
Sn	10.44	1.9540	336.2	120.1	38	336.2	138.1	38
Sp	7.20	1.0120	334.3	120.1	38	334.3	138.1	38
Sv	9.77	2.0300	336.3	120.1	41	336.3	138.2	41
Td	6.17	0.9880	354.3	120.1	45	354.3	222.3	38
7-Ly	8.41	0.4200	342.2	120.1	9	342.2	198.1	9
7-Im	8.15	0.4098	342.3	120.1	10	342.3	198.2	10
EmNO	13.00	2.0680	414.3	220.1	42	414.3	352.0	35
ErNO	3.74	1.0085	366.1	118.1	42	366.1	120.1	42
EuNO	4.54	1.0085	346.3	172.1	8	346.3	111.1	8
HeNO	8.81	1.0270	330.2	172.2	40	330.2	111.1	55
IcNO	5.15	0.3958	316.2	172.1	38	316.2	138.2	39
ImNO	5.15	0.4240	316.2	172.1	6	316.2	138.0	6
JbNO	4.24	0.9975	368.3	120.2	45	368.3	296.3	35
LcNO	13.70	0.3936	428.2	254.2	40	428.2	137.2	45
LyNO	5.15	0.9670	316.2	172.1	40	316.2	138.0	40
McNO	3.25	1.0310	342.1	118.1	50	342.1	137.1	40
ReNO	6.86	4.0980	368.1	118.1	45	368.1	136.0	40
SnNO	11.41	2.0230	352.2	118.1	40	352.2	136.0	45
SpNO	8.18	0.9735	350.2	120.1	42	350.2	138.1	38
SvNO	10.43	2.0210	352.2	118.1	42	352.2	136.1	42

¹ For the abbreviations of the compounds, refer to Section 2.1 (Standards). ² Determined using the UPLC conditions. ³ CE: collision energy.