Chemometric Methods for Simultaneous Determination of Candesartan Cilexetil and Hydrochlorothiazide in Binary Combinations

Table 3

Limit of detection (LOD) and limit of quantification (LOQ) of CAN by HPSAM.

λ	Regression equation	R^2	Added (µg/mL)		Found (µg/mL)
			HCT	CAN	HCT	CAN
283	Y = 0.01152X + 0.4192	0.9905	15	15	15.2	0.07
239	Y = 0.03433X + 0.4208	0.9962
283	Y = 0.01103X + 0.4202	0.9944	15	15	15.13	0.33
239	Y = 0.03432X + 0.4278	0.9925
283	Y = 0.01245X + 0.4128	0.9904	15	15	15.02	0.05
239	Y = 0.03637X + 0.4116	0.9945
283	Y = 0.01103X + 0.4154	0.9944	15	15	15.03	0.14
239	Y = 0.03386X + 0.4185	0.9922
283	Y = 0.01141X + 0.4182	0.9917	15	15	15.25	0.16
239	Y = 0.03510X + 0.4144	0.9977
Mean						0.15
SD						0.111
LOD						0.48
LOQ						1.26

¹ calculated using the regression equation of Y = 0.0054x + 0.0067 and the HCT calibration curve at 340 nm. ² calculated using the regression equation of Y = 0.0295x + 0.0648 and the HCT calibration curve at 250 nm.