Multicomponent Analysis of Liuwei Dihuang Pills by a Single Marker Quantification Method and Chemometric Discrimination of Fingerprints
Table 5
Relative retention times determined by different instruments and columns.
Instrument
Column
Relative retention time
RRT1
RRT2
RRT3
RRT4
RRT5
RRT6
RRT7
Waters e2695
1#
0.0860
0.1209
0.2722
0.5762
0.5964
0.6704
0.8736
2#
0.0947
0.1237
0.2826
0.5863
0.6065
0.6796
0.8752
3#
0.0954
0.1235
0.2851
0.5635
0.5978
0.6761
0.8742
Agilent 1260 InfinityII
1#
0.0873
0.1220
0.2739
0.5797
0.5993
0.6749
0.8759
2#
0.0956
0.1232
0.2848
0.5908
0.6090
0.6864
0.8777
3#
0.0957
0.1221
0.2847
0.5637
0.5956
0.6766
0.8761
Shimadzu Nexera X2
1#
0.0863
0.1178
0.2653
0.5710
0.5919
0.6674
0.8728
2#
0.0953
0.1208
0.2763
0.5823
0.6032
0.6780
0.8857
3#
0.0960
0.1211
0.2798
0.5614
0.5966
0.6768
0.8788
Mean
0.0925
0.1217
0.2783
0.5750
0.5996
0.6763
0.8766
RSD (%)
4.8
1.5
2.5
1.9
0.92
0.79
0.44
RRT1, RRT2, RRT3, RRT4, RRT5, RRT6, and RRT7 are the relative retention times of gallic acid, 5-hydroxymethylfurfural, morroniside, sweroside, loganin, paeoniflorin, and cornuside, respectively.
