Optimization of the Imaged cIEF Method for Monitoring the Charge Heterogeneity of Antibody-Maytansine Conjugate

Figure 1

Analysis of maytansinoid-antibody by icIEF. Experimental conditions: final concentration 1 mg/mL in 0.35% methyl cellulose, 2% pharmalytes 3–10 and 2% pharmalytes 8–10.5 (1 : 1 ratio), 2 M urea, pI markers: 6.61, 9.50. Focusing time: 10 min at 3000 V. λ = 280 nm.