Optimization of the Imaged cIEF Method for Monitoring the Charge Heterogeneity of Antibody-Maytansine Conjugate

Figure 8

icIEF profiles of naked antibody (a) and its corresponding maytansinoid conjugate (b). Final concentration of maytansinoid-antibody in sample matrix is 1 mg/ml diluted in 0.35% methyl cellulose, 4% 3–10 pharmalytes/8–10.5 pharmalytes (1 : 1 ratio), and 2 M urea, pI markers: 8. 18, 9.50 (a) and 6.61, 9.50 (b), and focusing time of 12 min at 3000 V. Other experimental conditions are as mentioned in Figure 1.

(a)

(b)