Optimization of the Imaged cIEF Method for Monitoring the Charge Heterogeneity of Antibody-Maytansine Conjugate

Table 1

An example of 200 μl sample preparation of ADC sample.

1 mg/ml	Volume μl	Final concentration
Methyl cellulose 1%	70	0.35%
Pharmalytes 3–10	4	2%
Pharmalytes 8–10.5	4	2%
Urea (8 M)	50	2 M
ADC 8 mg/ml	25	1 mg/ml
Water Milli-Q	45
pI marker 6.61	1
pI marker 9.5	1