Simultaneous Preconcentration of Fast Green FCF and Rhodamine B Using Deep Eutectic Solvent and Determination via High-Performance Thin Layer Chromatography

Table 3

Various mobile phases for simultaneous separation of fast green FCF and rhodamine B.

Number	Solvent system
1	Ethyl acetate (6.1 mL), methanol (2 mL), ammonia (0.925 mL), and deionized water (0.925 mL)
2	Ethyl acetate (6.1 mL), methanol (2 mL), and ammonia (0.925 mL)
3	Ethyl acetate (6.1 mL) and methanol (2 mL)
4	Ethyl acetate (6.1 mL), methanol (2 mL), formic acid (0.925 mL), and deionized water (0.925 mL)
5	Ethyl acetate (7.1 mL), methanol (1 mL), formic acid (0.925 mL), and deionized water (0.500 mL)
6	Ethyl acetate (7.1 mL), methanol (1.5 mL), formic acid (0.925 mL), and deionized water (0.500 mL)
7	Ethyl acetate (6.5 mL), methanol (2.5 mL), and formic acid (0.500 mL)
8	Ethyl acetate (8 mL) and formic acid (2 mL)
9	Ethyl acetate (6 mL), methanol (2 mL), and acetic acid (0.500 mL)
10	Ethyl acetate (6.1 mL), acetone (1 mL), ammonia (0.925 mL), and deionized water (0.925 mL)
11	Ethyl acetate (6.1 mL), acetone (3 mL), and formic acid (0.500 mL)
12	Ethyl acetate (9 mL), acetone (1 mL), ammonia (0.500 mL), and deionized water (0.200 mL)
13	Ethyl acetate (9.5 mL), acetone (0.500 mL), and formic acid (0.500 mL)
14	Ethyl acetate (9 mL), acetone (1 mL), and methanol (0.500 mL)
15	Ethyl acetate (9 mL), acetone (1 mL), methanol (0.500 mL), and deionized water (0.200 mL)
16	Ethyl acetate (6.1 mL), methanol (2 mL), and formic acid (0.925 mL)
17	Ethyl acetate (6 mL), methanol (2 mL), and ammonia (2 mL)

The bold value is used for the simultaneous separation of fast green FCF and rhodamine B.