Simultaneous and Visual Detection of KPC and NDM Carbapenemase-Encoding Genes Using Asymmetric PCR and Multiplex Lateral Flow Strip

Figure 8

Specificity investigation. (a) Genotyping of KPC and NDM using the multiplex strip. (b) Phenotyping of KPC and NDM enzymes based on drug susceptibility. (c) Detection of KPC and NDM enzymes using the antibody-based lateral flow immunochromatographic strip. (d) Detection of the KPC gene using real-time fluorescence PCR. (e) Detection of the NDM gene using real-time fluorescence PCR. Error bars represent the standard deviation of three independent measurements.

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