UVA Exposure Combined with Glycation of the Dermis Are Two Catalysts for Skin Aging and Promotes a Favorable Environment to the Appearance of Elastosis

Figure 1

Demonstration of the presence of AGEs and morphology of the reconstructed skin. Fluorescence measurement of collagen solution before reconstruction of the skin at λ_ex 335 nm/λ_em 385 nm (a) and λ_ex 370 nm/λ_em 440 nm (b). Control: native collagen solution; ribose 10 mM: collagen solution modified by glycation using ribose 10 mM (AU ± SD = arbitrary units ± standard deviation). Reconstructed skin (c): HES histological staining (A to D), vimentin (E to H), and AGEs (I to L) labelling of skin reconstructed in vitro using untreated collagen (A, B, E, F, I, J) or preglycated collagen (C, D, G, H, K, L) after (B, F, J, D, H, L) or without (A, E, I, C, G, K) UVA irradiation (10 J/cm²) (bar = 100 µm).

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