UVA Exposure Combined with Glycation of the Dermis Are Two Catalysts for Skin Aging and Promotes a Favorable Environment to the Appearance of Elastosis

Figure 3

AGEs and UVA irradiation change the elastic network homeostasis and promote an environment in favor of elastosis. mRNA MMP12 (a), elastase (ELANE) (b), and tropoelastin (ELN) (c) expression measured in dermal layers of reconstructed skin made with papillary fibroblasts and native or glycated collagen with or without UVA irradiation (0, 10 J/cm²); immunostainings (d) of fibrillin-1 (A, C) and tropoelastin (B, D) of skin reconstructed containing papillary fibroblasts (Fp) (A to D) using native collagen (A, B) or glycated collagen (C, D) after UVA irradiation (10 J/cm²). Note that glycation stimulates tropoelastin and fibrillin expression by fibroblasts in 3D culture. mRNA levels were quantified in the dermis (fibroblasts, n = 4) using qPCR at the end of the culture emersion phase. Data are expressed in arbitrary units (AU ± SD = arbitrary units ± standard deviation). Statistics: analysis of variance (ANOVA) adjusted post hoc Tukey–Kramer tests for pairwise comparisons (^∗P < 0.05; ^∗∗∗P < 0.0001).

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