Gene Position, base change, and rs# Genotyping
Primer PCR product Enzyme Gel band pattern p53 R72P G>C rs1042522 PCR-RFLP
-ATCTACAGTCCCCCTTGCCG-
(sense)
-GCAACTGACCGTGCAAGTCA-
(antisense)296 bp BstU IG allele: 169 bp, 127 bp C allele: 296 bp p53 Intron 3 16-bpins/del rs17878362 PCR
-TGGGACTGACTTTCTGCTCTT-
(sense)
-TCAAATCATCCATTGCTTGG-
(antisense)Del:
180 bp Ins:
196 bp p53 Intron 6 G>A
rs1625895 PCR-RFLP
-TGGCCATCTACAAGCAGTCA-
(sense)
-TTGCACATCTCATGGGGTTA-
(antisense)404 bp Msp IG allele: 336 bp, 68 bp A allele: 404 bp Bcl-2
938C>A
rs2279115PIRA-PCR: mismatch, antisense
primer +2 C-to-G
-TCCTGCCTTCATTTATCCAGCA-
(sense)
-CCAGGAGAGAGACAGGGGAC A-
(antisense)125 bp Nla IIIC allele: 106 bp, 19 bp A allele: 125 bp BAX
248G>A
rs4645878PIRA-PCR: mismatch, sense primer –2 G-to-C
-CATTAGAGCTGCGATTGGACC G-
(sense)
-GCTCCCTCGGGAGGTTTGGT-
(antisense)109 bp Msp IG allele: 89 bp, 20 bp A allele: 109 bp CASP9 Q221R G>A rs1052576 PIRA-PCR: mismatch, sense primer –2 G-to-C
-GGCTTTGCTGGAGCTGGCC C-
(sense)
-AGTACCCAATGCCTGCCCAGGG-
(antisense)121 bp Msp IG allele: 102 bp, 19 bp A allele: 121 bp DR4 T209R C>G
rs4871857 PCR-RFLP
-ATCCTCTGGGAACTCTGTGG-
(sense)
-GGGGACAGGCAGATGGAC-
(antisense)300 bp Dra IIIC allele: 200 bp, 100 bp G allele: 300 bp Fas
1377G>A
rs2234767PIRA-PCR: mismatch, sense primer –2 A-to-G
-TGTGTGCACAAGGCTGGCG C-
(sense)
-TGCATCTGTCACTGCACTTACCACCA-
(antisense)122 bp BstU IG allele: 104 bp, 18 bp A allele: 122 bp Fas –670A>G
rs1800682 PCR-RFLP
-ATAGCTGGGGCTATGCGATT-
(sense)
-CATTTGACTGGGCTGTCCAT-
(antisense)193 bp ScrF IA allele: 193 bp G allele: 136 bp, 57 bp FasL
844T>C
rs763110PIRA-PCR: mismatch, sense primer –7 A-to-C
-CAATGAAAATGAAC ACATTG-
(sense)
-CCCACTTTAGAAATTAGATC-
(antisense)85 bp Dra IIIT allele: 66 bp, 19 bp G allele: 85 bp CASP8 D302H G>C
rs1045485 PIRA-PCR: mismatch, sense primer –2 A-to-G
-CATTTTGAGATCAAGCCCCG C-
(sense)
-CCCTTGTCTCCATGGGAGAGGA-
(antisense)132 bp BstU IG allele: 112 bp, 20 bp C allele: 132 bp CASP10 I522L A>T
rs13006529 PIRA-PCR: mismatch,
antisense primer +4 A-to-T
-GAGTGGACAAACAGGGAACAAA-
(sense)
-AGAACCAACAAAAACTCTCTGCA ATA-
(antisense)122 bp Ssp IT allele: 97 bp, 25 bp A allele: 122 bp CASP3
1337C>G
rs1405937PIRA-PCR: mismatch, sense primer –2 C-to-A
-ATAGGCGCAAGTGTTAGAAACAGGA T-
(sense)
-CACCAACACATGTGAGCACGAC-
(antisense)112 bp Fok IC allele: 112 bp G
allele: 76 bp, 36 bp CASP7 D255E C>G
rs2227310 PIRA-PCR: mismatch,
sense primer –2 G-to-C
-CCTGGAGGAGCACGGAAAAC A-
(sense)
-TGGAGACCACACAGGGGATCTG-
(antisense)137 bp Pvu IIG allele: 115 bp, 22 bp C allele: 137 bp