Muscle Relaxant and Sedative-Hypnotic Activities of Extract of Viola betonicifolia in Animal Models Supported by Its Isolated Compound, 4-Hydroxy Coumarin

Table 1

Effects of VBME (%) on muscle relaxation (Chimney test and Traction test).

Group	Dose/kg	Chimney test (%)			Traction test (%)
		30 min	60 min	90 min	30 min	60 min	90 min
Control	10 mL	0 ± 0.00	0 ± 0.00	0 ± 0.00	0 ± 0.00	0 ± 0.00	0 ± 0.00
Diazepam	1 mg	100 ± 0.00***	100 ± 0.00***	100 ± 0.00***	100 ± 0.00***	100 ± 0.00***	100 ± 0.00***
VBME	0.3 g	10.12 ± 0.88	14.11 ± 0.97	11.09 ± 2.11	12.54 ± 1.10	15.23 ± 2.13	10.34 ± 1.90
	0.4 g	55.13 ± 1.23*	61.56 ± 0.65*	58.23 ± 0.56*	65.02 ± 0.23*	72.12.04 ± 0.56*	68.04 ± 0.34*
	0.5 g	77.08 ± 0.11**	80.76 ± 0.02**	80.55 ± 0.21**	75.05 ± 0.12**	78.03 ± 0.08**	77.67 ± 0.00**

Values represent the percentages of mice (𝑛=6) showing negative effects in Chimney and Traction tests, 30, 60, and 90 min after treatment with distilled water (10 mL/kg), VBME (0.3, 0.4, and 0.5 g/kg), or diazepam (1 mg/kg). Data are presented as mean ± S.E.M (𝑛=6). ^∗𝑃<0.05, ^∗∗𝑃<0.01, ^∗∗∗𝑃<0.001, all compared with control.