Muscle Relaxant and Sedative-Hypnotic Activities of Extract of Viola betonicifolia in Animal Models Supported by Its Isolated Compound, 4-Hydroxy Coumarin

Table 2

Effects of VBME open field test (sedative effect).

Treatment	Dose	No. of lines crossed in 10 min
Distilled water	10 mL/kg	126 ± 1.23
VBME	0.3 mg	90 ± 1.72
	0.4 mg	70 ± 1.67*
	0.5 mg	30 ± 0.89**
Diazepam	0.5 mg	5 ± 0.02***

Values represent the number of lines crossed by animal in open field, 30 min after treatment with distilled water (10 mL/kg), VBME (0.3, 0.4, and 0.5 g/kg), or diazepam (0.5 mg/kg). Data are presented as mean ± S.E.M (𝑛=6). ^∗𝑃<0.05, ^∗∗𝑃<0.01, ^∗∗∗𝑃<0.001, all compared with control.