Muscle Relaxant and Sedative-Hypnotic Activities of Extract of Viola betonicifolia in Animal Models Supported by Its Isolated Compound, 4-Hydroxy Coumarin

Table 3

Effect of VBME in phenobarbitone-induced sleep (hypnotic test).

Treatment	Dose (i.p.)	Onset of sleep (min)	Duration of sleeping (min)
Distilled water	10 mL/kg	25.12 ± 1.25	7.34 ± 2.28
Diazepam	4 mg/kg	5.45 ± 0.08***	56.45 ± 0.00***
VBME	0.3 g/kg	23.45 ± 0.87	8.13 ± 0.97
	0.4 g/kg	15.78 ± 0.78*	13.98 ± 1.76*
	0.5 g/kg	10.98 ± 0.91**	25.23 ± 1.46**

Values represent the onset of sleep and duration of sleep (in min) after treatment with distilled water (10 mL/kg), VBME (0.3, 0.4, and 0.5), or diazepam (4 mg/kg) and then all groups were treated with phenobarbitone (35 mg/kg). Data are presented as mean ± S.E.M (𝑛=6). ^∗𝑃<0.05, ^∗∗𝑃<0.01, ^∗∗∗𝑃<0.001, all with respect to control.