Muscle Relaxant and Sedative-Hypnotic Activities of Extract of Viola betonicifolia in Animal Models Supported by Its Isolated Compound, 4-Hydroxy Coumarin

Table 5

Effects of 4HC (%) on muscle relaxation (Chimney test and Traction test).

Group	Dose/kg	Chimney test (%)			Traction test (%)
		30 min	60 min	90 min	30 min	60 min	90 min
Control	10 mL	0 ± 0.00	0 ± 0.00	0 ± 0.00	0 ± 0.00	0 ± 0.00	0 ± 0.00
Diazepam	1 mg	100 ± 0.00***	100 ± 0.00***	100 ± 0.00***	100 ± 0.00***	100 ± 0.00***	100 ± 0.00***
4HC	10 mg	0 ± 0.00	0 ± 0.00	0 ± 0.00	0 ± 0.00	0 ± 0.00	0 ± 0.00
	20 mg	15.13 ± 1.34*	20.21 ± 1.35*	20.45 ± 0.44*	4.50 ± 1.67*	6.55 ± 1.22*	3.54 ± 1.89*
	30 mg	30.34 ± 1.54**	37.33 ± 1.57**	35.45 ± 1.45**	25.34 ± 0.23**	27.04 ± 1.77**	26.78 ± 2.56**

Values represent the percentages of mice (𝑛=6) showing negative effects in Chimney and Traction tests, 30, 60, and 90 min after treatment with distilled water (10 mL/kg), 4HC (10, 20, and 30 mg/kg), or diazepam (1 mg/kg). Data are presented as mean ± S.E.M, (𝑛=6). ^∗𝑃<0.05, ^∗∗𝑃<0.01, ^∗∗∗𝑃<0.001, all compared with control.