Muscle Relaxant and Sedative-Hypnotic Activities of Extract of Viola betonicifolia in Animal Models Supported by Its Isolated Compound, 4-Hydroxy Coumarin

Table 6

Sedative effect of 4HC in open field test.

Treatment	Dose	No. of lines crossed in 10 min
Distilled water	10 mL/kg	126 ± 1.23
4HC	10 mg	120.14 ± 1.65
	20 mg	122.37 ± 1.43
	30 mg	120.34 ± 1.12
Diazepam	0.5 mg	5 ± 0.02***

Values represent the number of lines crossed by animal in open field, 30 min after treatment with distilled water (10 mL/kg), 4HC (10, 20, and 30 mg/kg), or diazepam (0.5 mg/kg). Data are presented as mean ± S.E.M (𝑛=6). ^∗𝑃<0.05, ^∗∗𝑃<0.01, ^∗∗∗𝑃<0.001, all compared with control.